Analgesic and Anti-inflammatory Potential in Misopates orontium

Analgesic and Anti-inflammatory Potential of Misopates Orontium

Authors

  • Muhammad Ajaib Department of Botany, Mirpur University of Science and Technology (MUST), Mirpur-10250 (AJK), Pakistan
  • Zel-E- Arooj Department of Botany, Mirpur University of Science and Technology (MUST), Mirpur-10250 (AJK), Pakistan
  • Khalid Mohammed Khan H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan, Department of Clinical Pharmacy, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 31441, Dammam, Saudi Arabia
  • Kanwal H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
  • Shahnaz Perveen PCSIR Laboratories Complex, Shahrah-e-Dr. Salimuzzaman Siddiqui, Karachi-75280, Pakistan
  • Shazia Shah H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

Keywords:

Misopates orontium (L.) Raf., anti-inflammatory, diclofenac sodium, analgesic, aspirin

Abstract

The Misopates orontium (L.) Raf has been reported for its medicinal importance in the literature. This plant has been studied for its antioxidant and hepatoprotective studies. In the current study the in vitro analgesic and anti-inflammatory potentials of ethanol extracts of an ethnomedicinal plant Misopates orontium (L.) Raf was evaluated in a dose-dependent manner. The analgesic activity was determined via hot plate, tail flicking/immersion methods, and paw licking in mice, whereas, the anti-inflammatory potential was investigated by using carrageenan-induced hind paw edema in albino rats. The doses of 100, 200, and 300 mg/kg concentrations were used. The extract of 300 mg/kg displayed significant results within the range of 22.4 ± 0.79 and 7.2 ± 0.83 at 1.5 h for hot plate and tail immersion methods, respectively, in comparison with the standard drug aspirin. In the paw licking method the most effective dose was 300 mg/kg at which the licking and biting were reduced within 30 minutes, i.e. 3 ± 0.58 at 10 min. In the anti-inflammatory activity, the expansion in paw volume was measured after carrageenan injection. Results indicated that after carrageenan injection the paw volume increased but after a few intervals of time, it again reduced, i.e. 6.17 ± 0.15 at 2 h as compared to standard drug diclofenac sodium. This study reveals the anti-inflammatory and analgesic effectiveness of this plant, as it is also used locally to reduce pain and inflammations. Further studies on the isolation of active metabolites from the ethanol extract of M. orontium may lead to the discovery of new and novel molecules which can be used as drug candidates.

References

P. Murugan., A. Rajesha., T. Athiperumalsami., and V.R. Mohan. Screening of certain ethnomedicinal plants for antibacterial activity. Ethnobotanical Leaflets 12: 433-438 (2008).

K.M. Khan., N. Ambreen., U.R. Mughal., S. Jalil., S. Perveen., M.I. Choudhary. 3-Formylchromones: Potential anti-inflammatory agents. European Journal of Medicinal Chemistry 45: 4058-4064 (2010).

M.P. Mazura., D. Susanti., and M.A. Rasadah. Antiinflammatory action of components from Melastoma malabathricum. Pharmaceutical Biology 45: 372-375 (2007).

W. L. Herbst, D. R. and Sohmer, S. H. Manual of the Flowering Plants of Hawai’i. Vol, 1 and 2, University of Hawai’i and Bishop Museum Press. Honolulu, USA (1999).

N.G. Vallianou., P. Gounari., A. Skourtis., J. Panagos., and C. Kazazis. Honey and its anti-inflammatory, antibacterial and antioxidant properties. General Medicine 2: 1-5 (2014).

M.I. Choudhary., Azizuddin., S. Jalil., S.A. Nawaz., K.M. Khan., R.B. Tareen., Atta-ur-Rahman. Antiinflammatory and lipoxygenase inhibitory compounds from Vitex agnus-castus. Phytochemical Research 23: 1336-1339 (2009).

M.C. Lanhers., J. Fleurentin., P. Dorfman., F. Mortier., and Pelt. Analgesic, antipyretic and anti-inflammatory properties of Euphorbia hirta. Planta Medica 57: 225-231 (1991).

A.K. Gupta., D. Parasar., A. Sagar., V. Choudhary., B.S. Chopra., R. Garg., N. Khatri., F. Tasleem., I. Azhar., S.N. Ali., S. Perveen., and Z.A. Mahmood. Analgesic and anti-inflammatory activities of Piper nigrum L. Asian Pacific Journal of Tropical Biomedicine 7: 461-468 (2014).

I.O. Ishola., A.J. Akindele., and O.O. Adeyemi. Analgesic and anti-inflammatory activities of Cnestis ferruginea Vahl ex DC (Connaraceae) methanolic root extract. Journal of Ethnopharmacology 135: 55-62 (2011).

K. J. Harkiss. (1973). Studies in the Scrophulariaceae–part. vii1 structure of the aerial parts of Antirrhinum orontium L. (misopates orontium (L.) raf.). Planta Medica, 23 (02), 182-190.

S. Akbar., S. Ishtiaq. (2020). Hepatoprotective effect of Misopates orontium in rats. Bangladesh Journal of Pharmacology, 15(3), 75-81.

A. E. Al-Snafi. (2015). The pharmacological Importance of Antirrhinum majus-A review. Asian Journal of Pharmaceutical Science & Technology, 5(4), 313-320.

H. Ullah., S. Saleem., W. Ahmad., M. Hanif., Z. Ali., M. Israr. (2020). Antibacterial Activity of Different Extract of Medicinal Plant Antirrihnum orontium. Journal of Tropical Pharmacy and Chemistry.

B.B. Kakoti., P. Pradhan., S. Borah., K. Mahato., and M. Kumar. Analgesic and anti-inflammatory activities of the methanolic stem bark extract of Nyctanthes arbor-tristis Linn. BioMed Research International 1: 1-6 (2013).

S.E. Lee., H.J. Hwang., J.S. Ha., H.S. Jeong., and J.H. Kim. Screening of medicinal plant extracts for antioxidant activity. Life Science 73, 167-179 (2003).

H. Hosseinzadeh., and H. M. Younesi. Anti-nociceptive and antiinflammatory effects of Crocus sativus L. Stigma and petal extracts in mice. BMC Pharmacology 2: 1-8 (2002).

M. Ajaib., S. Ishtiaq, and M.F. Siddiqui. Comparative Analgesic Evaluation of Different Parts of Himalrandia tetrasperma and Wendlandia exserta of family Rubiaceae After Induction of Pain in Mice. Pakistan Journal of Pharmaceutical Science 31: 2509-2514 (2018).

M. Ajaib., S. Fatima., Ishtiaq, S., S. Perveen, and S. Shah. Evaluation of Analgesic Activity of Nicotiana plumbajnifolia. Journal of the Chemical Society of Pakistan 39: 441-445 (2017).

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Published

2020-03-17

How to Cite

Ajaib, M., Arooj, Z.-E.-., Khan, K. M. ., Kanwal, Perveen, S. ., & Shah, S. (2020). Analgesic and Anti-inflammatory Potential in Misopates orontium: Analgesic and Anti-inflammatory Potential of Misopates Orontium. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 57(1), 69–74. Retrieved from https://ppaspk.org/index.php/PPAS-B/article/view/75

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Research Articles