Antibacterial and Antibiofilm Activity of Pullulanase from Paenibacillus macerans Against Urinary Catheter-Associated Pathogens
DOI:
https://doi.org/10.53560/PPASB(63-1)1129Keywords:
Pullulanase, Urinary Catheters, Prebiotic, Biofilm Inhibition, Paenibacillus Macerans, Enzyme Purification, Lactic Acid BacteriaAbstract
Urinary tract infections and urinary catheters are frequently related. Clinicians in underdeveloped nations often encounter urinary tract infections (UTIs), which are among the most prevalent bacterial illnesses. Selecting the appropriate empirical treatment for a patient may be aided by the results of area-specific surveillance studies that seek to identify the bacteria causing UTIs and their patterns of resistance. Pullulinase, an enzyme that hydrolyzes pullulin into maltotriose, panose, and maltooligosaccharides, is capable of inhibiting bacterial colonization and biofilm formation on urinary tract catheters. The goal of this work is to detect Paenibacillus macerans isolated from rhizosphere soil as pullulanase producer and to use the purified pullulanase as prebiotic, antibacterial, and antibiofilm agent. Using of modified pullulin-agar for screening pullulanase producers. Purification of pullulanase by DEAE-Cellulose A-50 column followed by sephadex G-150 column. Isolation of urinary catheters bacteria and using of pullulanase and detection biofilm formation on the catheters then applied of pullulanase as antibacterial and antibiofilm agent against urinary catheters bacteria in addition to enhance the growth of lactic acid bacteria. Eleven isolates of Paenibacillus macerans (41%) had showed an ability to produce pullulanase with different levels. Serial steps were used Pullulanase purification which included 70% ammonium sulfate saturation, DEAE-Cellulose A-50 ion exchange chromatography and sephadex G-100 gel filtration chromatography with a recovery yield of 22.9%, 8.11 fold of purification and specific activity 15.90 U/mg. Acinetobacter baumannii and Pseudomonas aeruginosa were the most predominant isolates in the urinary catheters. The purified pullulanase has a potential prebiotic characteristic to enhance lactic acid bacteria growth while allegedly impeding the development of pathogens and inhibiting their biofilm formation. Prebiotic properties are typically intended not only to reduce pathogen growth and biofilm formation, but to promote the growth of desirable and beneficial bacteria like lactic acid bacteria. In conclusion, pullulanase can be added to animal feed to increase digestibility and gastrointestinal health. It can also be used to cover catheters to assist avoid infections and bacterial colonization.
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