Effect of Papain Enzyme in Feed on Digestibility of Feed, Growth Performance, and Survival Rate in Post Larvaes of Freshwater Lobster [Cherax quadricarinatus (Von Martens, 1868)]

Effect of Papain Enzyme on Freshwater Lobster

Authors

  • Diana Rachmawati Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Diponegoro University, Jl. Prof. Soedarto, SH, Tembalang, Semarang, Central Java 50275 Indonesia
  • Asep Awaludin Prihanto Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University , Jl. Veteran, Malang, East Java 65145, Indonesia
  • Roy Hendroko Setyobudi Waste Laboratory - University of Muhammadiyah Malang, Jl. Raya Tlogomas No. 246, Malang, East Java 65114, Indonesia
  • Olga Anne Department of Natural Sciences, Faculty of Marine Technology and Natural Sciences of Klaipeda University, H. Manto g. 84, Klaipėda 92294, Lithuania

Keywords:

Artificial feed, Diet management, Diet efficiency, Nutrient absorption, Proteolytic enzyme

Abstract

The study aimed to find the impact of additional papain enzyme in feed digestibility, growth performance, and survival rate in post larvaes of freshwater lobster [Cherax quadricarinatus (Von Martens, 1868)]. There were five treatments with dosage A (0 % papain enzyme per kg feed), B (0.1 % papain enzyme per kg feed), C (0.2 % papain enzyme per kg feed), D (0.3 % papain enzyme per kg feed) and E (0.4 % papain enzyme per kg feed). Every treatment was repeated three times. The experiment used Completely Randomized Design. EFU (Efficiency of Feed Utilization), FCR (Feed Conversion Ratio), PER (Protein Efficiency Ratio), RGR (Relative Growth Rate), ADCP (Apparent Digestibility Coefficient of Protein), SR (Survival Rate) and water quality parameters were determined. The study found that the impacts of the various dosages of papain enzyme exhibited significant effects (P < 0.01) on the EFU, FCR, PER, RGR, ADCP; however, insignificant effects were observed (P > 0.05) on the survival rate of freshwater lobster. It was concluded that the incorporation of papain enzyme improved the feed digestibility and the growth performance of post larvae in freshwater lobster. The study found that optimum dosages of papain enzyme on feed digestibility, efficiency of feed utilization and growth rate of freshwater lobster ranged from 0.24 % to 0.31 % papain enzyme per kg feed. The quality of media culture was still in the feasible condition for post larvae of freshwater lobster cultivation.

References

Akiyama, D.M., W.G. Domny & A.L. Lawrence. Penaeid shrimp nutrition. In: Marine Shrimp Culture: Principles and Practices. Fast, A.W. &

L.J. Lester (Ed.). Elsevier Publication, Amsterdam, 535–568 (1992).

Chung, T.K. Sustaining Livestock Production and Environment. Food and Agriculture Asia Pacific Development, Singapore. pp. 52‒54 (2001).

Singh, P, M. Sajid, H.S. Munir, P. Vikas, M. Danish, Ramesh & C. Singh. Exogenous supplementation of papain as growth promoter in diet of fingerlings of Cyprinus carpio. International Aquatic Research 3: 1–9 (2011).

Gul, Y., M. Salim & B. Rabbani. Evaluation of apparent digestibility coefficient of different dietary protein level with and without fish meal for Labeo rohita. Pakistan Veterinary Journal 27(3): 121–125 (2007).

Choi, W.M., W.Y. Lam, W.Y. Mo & M.H. Wong. Upgrading Food Wastes by Means of Bromelain and papain to enhance growth and immunity of grass

carp (Ctenopharyngodon idella). Environmental

Science and Pollution Research 23(8): 7186‒7194

(2016).

Farhangi, M. & C.G. Carter. Effect of enzyme

supplementation to dehulled lupin-based diets on

growth, feed efficiency, nutrient digestibility and

carcass composition of rainbow trout Oncorhynchus

mykiss (Walbaum). Aquaculture Research 38(12):

‒1282 (2007).

Mo, W.Y., R.S.S. Lau, A.C.K. Kwok & M.H.

Wong. Use of soybean meal and papain to partially

replace animal protein for culturing three marine

fish species: Fish growth and water quality.

Environmental Pollution 219: 815–820 (2016).

Panigrahi, A., V. Kiron, J. Puangkaew, T. Kobayashi, S. Satoh & H. Sugita. The viability of probiotic bacteria as a factor influencing the immune response in Rainbow trout Oncorhynchus mykiss. Aquaculture 243: 241‒254 (2005).

Shelby, R.A., C. Lim, M. Yildirim & P.H. Klesius. Effects of probiotic bacteria as dietary supplements on growth and disease resistance in young channel catfish, Ictalurus punctatus (Rafinesque). Journal of Applied Aquaculture 18(2): 49‒60 (2006).

Patil, D.W. & H. Singh. Effect of papain supplemented diet on growth and survival of postlarvae of Macrobrachium rosenbergii. International Journal of Fisheries and Aquatic Studies 1(6): 176‒179 (2014).

Khati. A., M. Danish, K.S. Mehta1 & N.N. Pandey. Estimation of growth parameters in fingerlings of Labeo rohita (Hamilton, 1822) fed with exogenous nutrizyme in Tarai region of Uttarakhand, India. African Journal of Agricultural Research 10(30): 3000–3007 (2015).

Muchlisin, Z.A., A. Fardin, M. Tanzil, F. Nur, J. Zulkarnain, A.A. Muhammadar, et al. The effectiveness of experimental diet with varying

levels of papain on the growth performance, survival rate and feed utilization of Keureling Fish (Tor tambra). Biosaintifika 8 (2): 172–177 (2016).

Pavosovic, A. Evaluation of the nutritional requirements of redclaw crayfish Cherax quadricarinatus. [Dissertation] Queensland

University of Technology, pp. 1‒179 (2008).

National Research Council. Nutrient Requirements of Fish and Shrimp. Washington, DC: The National Academies Press. (2011).

AOAC. Official Methods of Analysis 20th Edition. Association of Official Analytical Chemists, Washington D.C., pp. 1298 (2016).

Aurand, L.W., A.E Woods & M. R. Wells. Food Composition and Analysis. Springer Science Bussines Media LLC, 665 p (2013).

Tacon, A.G.J., J.J. Cody., L.D. Conquest, S. Divakaran,I.P. Forster, & O.E. Decamp. Effect of culture system on the nutrition and growth

performance of Pacific white shrimp Litopenaeus vannamei (Boone) fed different diets. Aquaculture Nutrition 8: 121–137 (2002).

Steel, R.G.D., J.H. Torrie & D.A. Dickey. Principles and Procedures of Statistics: A Biometrical Approach. 3rd Edition, McGraw Hill, Inc. Book Co., New York, 352–358 (1997).

Sajjadi, M & C.G. Carter. Effect of phytic acid and phytase on feed intake, growth, digestibility and trypsin activity in Atlantic salmon (Salmo salar, L.). Aquaculture Nutrition 10(2):135–142 (2004).

Gatlin III, D.M. Principles of fish nutrition. Regional Aquaculture Center Publication No. 5003 (2010).

Mo, W.Y., R.S.S. Lau, A.C.K. Kwok & M.H. Wong. Use of soybean meal and papain to partially replace animal protein for culturing three marine fish species: Fish growth and water quality. Environmental Pollution 219: 815–820 (2016).

Manush, S.M., P.P. Srivastava, M.P.S. Kohli, K.K. Jain, S. Ayyappan, & S.Y. Metar. Combined effect of papain and vitamin-C levels on growth performance of Freshwater Giant Prawn, Macrobrachium rosenbergii. Turkish Journal of Fisheries and Aquatic Sciences 13: 479–486 (2013).

Miyamoto, D., J. Watanabe & K. Ishihara. Effect of water soluble phospholipids polymers conjugated with papain on the enzymatic stability. Biomaterials 25: 71‒76 (2004).

Dawood, M.A.O., A.E. Dakar, M. Mohsen, E. Abdelraouf, S. Koshio, M. Ishikawa, et al. Effects of using exogenous digestive enzymes or natural enhancer mixture on growth, feed utilization, and body composition of rabbitfish, Siganus rivulatus. Journal Agriculture Science and Technology 8(4): 180– 87 (2014).

Yakuputiyage, A. On-farm feeding and feed management strategies in tropical aquaculture. In: On-Farm Feeding and Feed Management in Aquaculture. Hasan M.R. & M.B. New (Ed.). FAO Fisheries and Aquaculture Technical Paper No. 583. Rome, FAO. pp. 361‒376 (2013).

Hepher. Nutrition of Pond Fishes. Cambridge University, Cambridge, p. 365 (1988).

Wilson, R.P. Energy relationships in catfish diets. In: Nutrition and Feeding of Channel Catfish. Stickney, R.R. & R.T. Lovell (Ed.). Southern Cooperative Series, Auburn, Alabama, pp. 193‒201 (1982).

De Silva, S.S. Culture-based fisheries: An underutilised opportunity in aquaculture development. Aquaculture 221 (1–4) : 221–243 (2003).

Boyd, C.E. Guidelines for aquaculture effluent management at the farm level. Aquaculture 226:101–112 (2003).

Downloads

Published

2018-09-17

How to Cite

Rachmawati, D. ., Prihanto, A. A. ., Setyobudi, R. H. ., & Anne, O. . (2018). Effect of Papain Enzyme in Feed on Digestibility of Feed, Growth Performance, and Survival Rate in Post Larvaes of Freshwater Lobster [Cherax quadricarinatus (Von Martens, 1868)]: Effect of Papain Enzyme on Freshwater Lobster. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 55(3), 31–39. Retrieved from https://ppaspk.org/index.php/PPAS-B/article/view/120

Issue

Vol. 55 No. 3 (2018)

Section

Research Articles

License

Creative Commons Attribution (CC BY). Allows users to: copy the article and distribute; abstracts, create extracts, and other revised versions, adaptations or derivative works of or from an article (such as a translation); include in a collective work (such as an anthology); and text or data mine the article. These uses are permitted even for commercial purposes, provided the user: includes a link to the license; indicates if changes were made; gives appropriate credit to the author(s) (with a link to the formal publication through the relevant DOI); and does not represent the author(s) as endorsing the adaptation of the article or modify the article in such a way as to damage the authors' honor or reputation.