Antidiabetic and Antimicrobial Properties of Some High Altitude Medicinal Plants of Nepal
Antidiabetic and Antimicrobial Properties of High Altitude Plants of Nepal
Keywords:Medicinal plants, Antimicrobial, Protein Tyrosine Phosphatase 1B, High altitude, Type 2 Diabetes Mellitus
Regarding severe side effects caused by synthetic drugs, studies were carried out taking high altitude medicinal plants to evaluate antidiabetic and antimicrobial activities. Antidiabetic property was measured by the inhibition protein tyrosine phosphatase 1B (PTP1B) enzyme with plant extract taking p-nitrophenyl phosphate (pNPP) as a substrate in the assay. For antimicrobial activity, plant extracts were tested against four pathogenic bacteria; Staphylococcus aureus, Escherichia coli, Klebisella pneumonia and Pseudomonas aeruginosa. BA17 appeared to be more effective for the inhibition PTP1B enzyme (95-100%) at various concentrations. BU17 showed the maximal zone of inhibition of 16 mm on three microbes in S. aureus, E. coli, and K. pneumonia at 100 mg/mL.
Baral S.R. & P.P Kurmi. A Compendium of Medicinal Plants in Nepal. American Journal of plants science 8: 6 (2006).
Bennett R.N. & R. M. Walls grove Secondary Metabolites in Plant Defense Mechanisms. Journal of New Phytology127:617-633(1994).
Bhattarai B. R., Kafle B., HwangJ. S., Khadka D., Lee S. M., Kang J. S., Ham S. W., Han I. O., Park H &, H. Cho. Thiazolidinedione derivatives as PTP1B inhibitors with anti-hyperglycemic and anti-obesity effects. Bioorganic & medicinal chemistry letters. 19(21) 6161-61(2009).
Bustanji Y., Taha M.O., Al-Masri I. M. & M. K. Mohammad. Docking simulations and in vitro assay unveil potent inhibitory action of papaverine against protein Tyrosine phosphatase 1B. Biology Pharmaceutical Bulletin. 32:640–5(2009).
Chen C., Zhang Y. & C. Huang. Berberine inhibits PTP 1B activity and mimics insulin action. J Biochem Biophys Res Commun. 397: 543-47(2010).
Doughari. J. H..Phytochemical extraction methods, basic extraction and mode of action of the potential chemotherapeutic agent. JINTECH3: 7-8 (2012).
Eells S., Mckinnell. J., Wang. A, Green N., Whang. O. & L. Miller. A comparison of clinical outcomes between healthcare-associated infections due to community-associated methicillin-resistant Staphylococcus aureus strains and health care associated methicillin-resistant S. aureus strains. Journal of Epidemiology and Infectious. 141(10) 2140-2148(2013).
Hammer. K. A., Carson C.F. & T.V. Riley. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology. 86 (6) 985(1999).
Hancock. R. E. W. Mechanisms of action of newer antibiotics for Gram-positive pathogens. Lancet Infectious Disease 5(4): 209–18(2005).
John. K.. M., Vijayan. D., Raj Kumar. R. R. & R. Premkumar. Factors influencing the efficiency of extraction of polyphenols from Young tea Leaves. Asian Journal of Plant Science 5: 123- 126(2006).
Kaul M. K. High altitude botanicals in integrative medicine. Case studies from Northwest Himalaya. Indian Journal of Traditional knowledge. 9(1)18-25 (2010).
Mandal S. C. & A.K. Das. Essentials of Botanical Extraction. Academic press. 63-82 (2015).
Panzhinskiy E., Ren J. & S. Nair. Pharmacological inhibition of protein tyrosine phosphatase 1B: A promising strategy for the treatment of obesity and Type 2 diabetes mellitus. Current Medicinal Chemistry 20:2609–25(2013).
Saidu Y., Muhammad S.A., Abbas A.Y., Onu A., Muhammad I. & L. Muhammad. In vitro screening for protein tyrosine phosphatase 1B and dipeptidyl peptidase IV inhibitors from selected Nigerian medicinal plants. Journal Intercutt Ethno pharmacol 6(2) 154-157(2016).
Srivastava J., Lambert J. & N. Vietmeyer. Medicinal plants: An expanding role in development World Bank Technical 320(1996).
Uniyal S.K., Singh. K. N., Jamwal P. & B. Lal. Traditional use of medicinal plants among the tribal communities of ChhotaBhangal, Western Himalayan. Journal Ethnobiol. Ethnomed 2:1-14(2006).
Yusuf S., Suleiman A. M., Abdullahi Y. A., Andrew O., Ibrahim M. T. & M. Luba. In vitro screening
for protein tyrosine phosphatase 1B and dipeptidyl peptidase IV inhibitors from selected Nigerian medicinal plants. Journal of Intercult Ethno pharmacol 6(2) 154-157 (2017).
Zengin G., Guler G. O., Aktumsek A., Ceylan R., Picot C. M. N. & M. F. Mahomoodally. Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey. Advances in Pharmacological Sciences 2015: 1-8 (2015).