Isolation, Purification and Quantification of Quercetin and Primary Metabolites from Onion (Allium cepa L.)
Isolation, Purification, Quantification of Quercetin from Onion
Keywords:
HPLC and GC analysis, phytochemicals, Allium cepa L,Abstract
Plants constitute a key component of drug in fold system, and thus are a source of inspiration for many
drugs. This study was conducted to quantify carbohydrates, proteins, lipids, metal ions and flavonoids in the bulbs
of three different varieties of Allium cepa L. Gas chromatography (GC) analysis was performed for analysing fatty
acids while high-performance liquid chromatography (HPLC) was employed for quantification of Quercetin. Results
of this investigation revealed that dark pink A. cepa contains higher concentration of cellulose (17.55%) and lower
concentration of oil (2.12 %) and its GC analysis showed that propionate concentration was much higher (31.61 %). Levels of protein and crude fat in the onion samples were 8.75% and 9.9%, respectively. Macro elements were observed in high concentration and sodium was highest (14.0 μg/mL); however, copper concentration was observed to be in traces (0.3 μg/ mL). The HPLC analysis of flavonoids revealed that A. cepa was rich in Quercetin-3, 4- O-diglucoside (3, 4- Qdg); Quercetin-3-O-glucoside (3-Qmg or iso quercitrin); Quercetin-4 -O-glucoside (4-Qmg); Isorhamnetin-3-O-glucoside (3-Img), Quercetin aglycone (Q); and Kaempferol (K) in which concentration of Quercetin-4 - O-glucoside (4 - Qmg) was significantly higher. The present study helped in purification of fatty acids and quercetin and for formulation of drugs which utilize these phytochemicals.
References
AOAC. Official Methods of Analysis of the AOAC, 15th ed. Methods 932.06, 925.09, 985.29, 923.03. Association of Official Analytical Chemists. Arlington, VA, USA (1990).
Benkeblia, N., S. Onodera, T. Yoshihira, S. Kosaka & N. Shiomi. Effect of temperature on soluble invertase activity, and glucose, fructose and sucrose status of onion bulbs (Allium cepa) in store. Interational Journal of Food Science and Nutrition 55: 325– 331 (2004).
Chope, G.A., K. Cools, J.P. Hammond, A.J. Thompson & L.A. Terry. Physiological, biochemical and transcriptional analysis of onion bulbs during storage. Journal of Annals of Botany 109: 819– 831 (2012).
Corzo-Martínez. M., N. Corzo & M. Villamiel. Biological properties of onions and garlic. Journal of Trends in Food Science and Technology 18 (12): 609–625 (2007).
Daniel, Z., & H. Maria, Domestication of Plants in the Old World (3rd ed.). Oxford: Oxford University Press: 198 (2000).
Elmuez, A. A. Aboagarib, R. Yang, X. Hua & A. Siddeeg. Chemical compositions, nutritional properties and volatile compounds of Guddaim (Grewia Tenax. Forssk) fiori fruits. Journal of Food and Nutrition Research 2 (4): 187-192 (2014).
Erwin, W., A. Gramza. , M. Heoe, H. Henryk, Jelen, J. Korczak, M. Maecka, S. Mildner-Szkudlarz, M. Rudziñska, U. Samotyja & R. Zawirska-Wojtasiak. Oxidation of lipids in food. Polish Journal of Food and Nutrition Science 13/ 54 (SI 1): 87– 100 (2014).
Hansen, S.L. Content and composition of dry matter in onion (Allium cepa L.) as influenced by developmental stage at time of harvest and longterm storage. Acta AgriculturaeScandinavica 49: 103– 109 (1999).
Knekt, K. Dietary flavonoids and the risk of lung cancer and other malignant neoplasm. American Journal of Epidemiologicals 146: 223- 230 (2002).
Lillian, B; F. Maria-Joao, Q. Bruno, Ferreira, C.E.R.F. Isabel & B. Paula. Total phenols, ascorbic acid, b-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chemistry 103: 413–419 (2007).
Lowry, O.H, N.J. Rosenburgh, A.L. Farr & R.J. Randall. Protein measurement with Folin Phenol Reagent. Journal of Biological Chemistry 193: 265- 275 (1951).
Nuutila A.M., K. Kammiovirta & K.M. Oksman- Caldentey. Comparison of methods for the hydrolysis of flavonoids and phenolic acids from onion and spinach for HPLC analysis. Journal of Food Chemistry 76 (4): 519– 525 (2002).
Patil, B.S., & L.M. Pike. Distribution of Quercetin content in different rings of various colored onion cultivars. Journal of Horticulture Studies 70: 643- 650 (1995).
Perchonok, M. & C. Bourland. NASA food systems: past, present and future. Nutrition 18: 913– 920(2002).
Price, K.R. & M.J. .C. Rhodes. Analysis of the major flavonol glycosides present in four varieties of onion and changes in composition resulting from autolysis. Journal of Food and Agriculture 74: 331-339 (1997).
Wattel, A., S. Kamel, R. Mentaverri, F. Lorget, C. Prouillet, J. Petit, Patrice, Fardelonne & M. Brazier. Potent inhibitory effect of naturally occurring flavonoids quercetin and kaempferol on in vitro osteoclastic bone resorption. Journal of Biochemical Pharmacology 65 (1): 35– 42 (2003).
Xing, N.Y., S.H. Chen, Mitchell & C.Y.F. Young. Quercetin inhibits the expression and function of the androgen receptor in LNCaP prostate cancer cells. Carcinogenesis 22 (3): 409- 414 (2001).
Zoltan, M., M. Angelone, C. Brunori & R. Morabito. Digestion methods for analysis of fly ash samples by atomic absorption spectrometry. Journal of Analytical Chimical Acta 395 (1- 2):157-163 (1999).
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