Evaluation of Biological Activity of Crude Extracts from Plants used by Indigenous Communities of Pothohar Plateau, Pakistan
DOI:
https://doi.org/10.53560/PPASB(60-4)922Keywords:
Moringa, Pothohar, Plateau, Antimicrobial, AntioxidantAbstract
The present study aimed to evaluate the potential of eight plant extracts that are used by communities of the Pothohar Plateau. Selected plants were Brassica campestris, Brassica oleracea var. italica, Allium sativum, Piper nigrum, Cinnamomum zeylanicum, Allium cepa, Olea europaea and Moringa oleifera. The antimicrobial assessment was carried out by using the agar diffusion method and antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl free radical assay, phosphomolybdate assay, and reducing power assay, against the selected isolates. Antimicrobial and growth rate studies were carried out by using two Gram-negative, one Gram-positive, and two pathogenic fungal strains. Among all tested extracts M. oleifera appeared to have the highest bioactivity with a percentage inhibition of 89 % against DPPH free radical, followed by Allium sativum 81 %, Allium cepa 75 %, Olea europaea 67 %, B. campestris 60 %, B. oleracea 58 %. During the phosphomolybdate assay similar trends were obtained such as: M. oleifera 91 % followed by A. sativum 85 %, A. cepa 78 %, Brassica campestris 72 %, Brassica oleracea 70 % and Olea europaea 65 % in higher concentration (1000 µg/ml). In the case of antibacterial assay Moringa oleifera showed maximum zone of inhibition against Staphylococcus aureus (20mm) followed by Klebsiella pneumonia (18 mm) and Escherichia coli, (17mm) whereas, crude extract of Allium sativum showed maximum zone of inhibition 14.4 mm against A. flavus whereas M. oleifera gave maximum zone of inhibition against A. alternata, followed by A. sativum and A. cepa. All the tested extracts showed bioactivities. This study can indicate the antimicrobial and antioxidant potentials of M. oleifera, and A. species. Hence, it is recommended that the extracts of these plants should be further evaluated for their possible application as antimicrobial and antioxidant agents.
References
M. Ozturk, V. Altay, K.R. Hakeem, and E. Akcicek (Eds.). Pharmacological Activities and Phytochemical Constituents. In: Liquorice From Botany to Phytochemistry. Springer pp. 45-47 (2017).
T. Goswami. Religion and Medicine in Ancient India: A Different Discourse Based on Caraka Saṃhitᾱ. Marburg Journal of Religion 22(2): 1-25 (2020).
R.T. Mertens, S. Gukathasan, A.S. Arojojoye, S.C. Olelewe, and S.G. Awuah. Next Generation Gold Drugs and Probes: Chemistry and Biomedical Applications. Chemical Reviews 123(10): 6612-6667 (2023).
M. Ozturk, and K.R. Hakeem. Plant and Human Health - Vol. 2 - Phytochemistry and Molecular Aspects. Springer Science Business Media NY (2019a).
A. Manzoor, B. Yousuf, J.A. Pandith, and S. Ahmad. Plant-derived active substances incorporated as antioxidant, antibacterial or antifungal components in coatings/films for food packaging applications. Food Bioscience 102717 (2023).
M.K.A. Ansari, B.T. Unal, M. Ozturk, and P. Owens (Eds.). Plants as Medicine and Aromatics. Pharmacognosy, Ecology and Conservation. CRC Press (2023).
P. Choudhary, T.B. Devi, S. Tushir, R.C. Kasana, and D.S. Popatrao. Mango seed kernel: A bountiful source of nutritional and bioactive compounds. Food and Bioprocess Technology 16(2): 289-312 (2023).
M. Pateiro, R. Dominguez, P.E. Munekata, G. Nieto, S.P. Bangar, K. Dharma, and J.M. Lorenzo. Bioactive compounds from leaf vegetables as preservatives. Foods 12(3): 637 (2023).
Y. Qin, Y. Liu, L. Yuan, H. Yong, and J. Liu. Preparation and characterization of antioxidant, antimicrobial, and pH-sensitive films based on chitosan, silver nanoparticles, and purple corn extract. Food Hydrocolloids 96: 102-111 (2019).
K. Hara, T. Someya, K. Sano, Y. Sagane, T. Watanabe, and R.G.S. Wijesekara. Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay. Data in Brief 17: 870-875 (2018).
A. Gupta, R. Kumar, and A.K. Pandey. Antioxidant and antidiabetic activities of Terminalia bellirica fruit in alloxan-induced diabetic rats. South African Journal of Botany 130: 308-315 (2020).
E. Dilshad, M. Bibi, N.A. Sheikh, K.F. Tamrin, Q. Mansoor, Q. Maqbool, and M. Nawaz. Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity. Journal of Functional Biomaterial 11(4): 76 (2020).
R.L. Prior, X. Wu, and K. Schaich. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of agricultural and food chemistry 53(10): 4290-4302 (2005).
N. Bhalla, N. Ingle, S.V. Patri, and D. Haranath. Phytochemical analysis of Moringa oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications. Saudi Journal of Biological Sciences 28(12): 6915-6928 (2021).
J. Khunchalee, and V. Surapat. The study of free radical scavenging, total phenolic contents, and tyrosinase inhibition activity of crude extract from Moringa oleifera Lam. Creative Science 15(1): 241942-241942 (2023).
A.J. Chakraborty, T.M. Uddin, B.M. R. Zidan, and S. Mitra. Allium cepa: a treasure of bioactive phytochemicals with prospective health benefits. Evidence-Based Complementary and Alternative Medicine 18: 4586318 (2022).
N. Marefati, V. Ghorani, and F. Shakeri. A review of anti-inflammatory, antioxidant, and immunomodulatory effects of Allium cepa and its main constituents. Pharmaceutical Biology 59(1): 287-302 (2021)..
D.J. Cherubim, C.V. Martins, L. Fariña, and R.A. Lucca. Polyphenols as natural antioxidants in cosmetics applications. Journal of Cosmetic Dermatology 19(1): 33-37(2020).
A.V. Khan, Q.U. Ahmed, M.R. Mir, I. Shukla, and A.A. Khan. Antibacterial efficacy of the seed extracts of Melia azedarach against some hospital-isolated human pathogenic bacterial strains. Asian Pacific Journal of Tropical Biomedicine 1(6): 452-455 (2011).
M. Awasthi, C.P. Pokhrel, Y.H. You, S. Balami, R.M. Kunwar, S. Thapa, E.J. Kim, J.W. Park, J.H. Park, J.M. Lee, and Y.S. Kim. Comparative assessment of ethnobotany and antibacterial activity of Moringa oleifera Lam. in Nepal. Ethnobotany Research and Applications 25: 14 (2023).
Miladiarsi, N. Basir, Nurlindasari, Wahdaniar, and A. Irma. Antibacterial activity test of Moringa leaf ethanol extract ointment of Moringa oleifera Lamk. on Staphylococcus aureus bacteria. Journal of Health Sciences and Medical Development 2(01): 13-19 (2023).
O.M. Oyawoye, T.M. Olotu, and S.C. Nzekwe, J.A. Idowu, T.A. Abdullahi, S.O. Babatunde, I.A. Ridwan, G.E. Batiha, N. Idowu, M. Alorabi, and H. Faidah. Antioxidant potential and antibacterial activities of Allium cepa (onion) and Allium sativum (garlic) against the multidrug resistance bacteria. Bulletin of the National Research Centre 46(1): 1-7 (2022).
N. Benkeblia. Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). LWT-Food Science and Technology 37(2): 263-268 (2004).
N. Marefati, V. Ghorani, and F. Shakeri. A review of anti-inflammatory, antioxidant, and immunomodulatory effects of Allium cepa and its main constituents. Pharmaceutical Biology 59(1): 287-302 (2021).
A.P. Pereira, I.C.F.R. Ferreira, F. Marcelino, P. Valentão, P.B. Andrade, R. Seabra, L. Estevinho, A. Bento, and J.A. Pereira. Phenolic Compounds and Antimicrobial Activity of Olive (Olea europaea L. Cv. Cobrançosa) Leaves. Molecules 12(5): 1153-1162 (2007).
A.D.T. Phan, G. Netzel, P. Chhim, M.E. Netzel, and Y. Sultanbawa. Phytochemical characteristics and antimicrobial activity of Australian grown garlic (Allium sativum L.) cultivars. Foods 8(9): 358 (2019).
M. Marrelli, V. Amodeo, G. Statti, and F. Conforti. Biological properties and bioactive components of Allium cepa L.: Focus on potential benefits in the treatment of obesity and related comorbidities. Molecules 24(1): 119 (2018).
M.K. Agrawal, D. Rathore, S. Goyal, A. Varma, and A. Varma. Antibacterial efficacy of Brassica campestris Root, Stem and Leaves extracts. International Journal of Advanced Research 1(5): 131-135 (2013).
R.D. Pacheco‐Cano, R. Salcedo‐Hernández, J.E. López‐Meza, D.K. Bideshi, and J.E. Barboza-Corona. Antimicrobial activity of broccoli (Brassica oleracea var. italica) cultivar Avenger against pathogenic bacteria, phytopathogenic filamentous fungi, and yeast. Journal of Applied Microbiology 124(1): 126-135 (2018).
R.K. Saini, I. Sivanesan, and Y.S. Keum. Phytochemicals of Moringa oleifera: a review of their nutritional, therapeutic and industrial significance. Biotechnology 6(2): 1-14 (2016).
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