Investigating the Burden of Pseudomonas aeruginosa Infections in COVID Patients and Resistance Profiles in Abbottabad, Pakistan
DOI:
https://doi.org/10.53560/PPASB(61-sp1)1007Keywords:
Antimicrobial Resistance, Co-infection, Moxifloxacin, Pseudomonas aeruginosa, VancomycinAbstract
Nosocomial infections are a great menace for hospitalized patients and Pseudomonas aeruginosa has emerged as one of the most potent nosocomial pathogens due to its continuous emanation of multi-drug resistance. Conjointly, P. aeruginosa coinfection is a significant problem in multitudinous infections. The present study aimed to investigate the frequency and antibiotic susceptibility profile of P. aeruginosa, from the specimens isolated from patients from Ayub Teaching Hospital in Abbottabad, Pakistan. In this cross-sectional study, eighty strains of P. aeruginosa were obtained from 200 patients (urine, wounds, and pus samples), using routine microbiological methods, and antibiotic susceptibility testing was performed using the Kirby Bauer disc diffusion method. The majority of isolates (51.25%) were taken from wounds, followed by pus (38%) and urine (27.27%). Of the 80 isolates, 12 originated from individuals who were also infected with the Coronavirus (a 34% coinfection rate). These isolates were sensitive to levofloxacin (80%) and vancomycin (75%) but were resistant to moxifloxacin (80%) and amikacin (69%). P. aeruginosa is found in high frequency in clinical specimens from patients in Abbottabad, and these microorganisms are transiently resistant to routinely given antibiotics, making it critical to utilize anti-Pseudomonas medications correctly. It is concluded that the P. aeruginosa infections and resistance continued to increase owing to various intrinsic and extrinsic factors.
References
1. D. Subedi, A.K. Vijay, and M. Willcox. Overview of mechanisms of antibiotic resistance in Pseudomonas
aeruginosa: an ocular perspective. Clinical and Experimental Optometry 101: 162-171 (2018).
2. S. Zahoor, H.M. Mahboob, H. Bukhari, A. Ali, H.M.S. Jehangir, and A. Siddiqui. Frequency and Antimicrobial Susceptibility Pattern of Pseudomonas aerugonisa Clinical Isolates at a Large Teaching Hospital In Lahore, Pakistan. Pakistan Postgraduate Medical Journal 31: 168-171 (2020).
3. L. Lansbury, B. Lim, V. Baskaran, and W.S. Lim. Co-infections in people with COVID-19: a systematic
review and meta-analysis. Journal of Infection 81: 266-275 (2020).
4. S.J. Cole, S.J., A.R. Records, M.W. Orr, S.B. Linden, and V.T. Lee. Catheter-associated urinary tract infection by Pseudomonas aeruginosa is mediated by exopolysaccharide-independent biofilms. Infection and Immunity 82: 2048-2058 (2014).
5. H. Mahmoudi. Bacterial co-infections and antibiotic resistance in patients with COVID-19. GMS Hygiene
and Infection Control 15: (2020).
6. E. Sharifipour, S. Shams, M. Esmkhani, J. Khodadadi, R. Fotouhi-Ardakani, A. Koohpaei, Z. Doosti, and S.EJ Golzari. Evaluation of bacterial co-infections of the respiratory tract in COVID-19 patients admitted to ICU. BioMed Central Infectious Diseases 20: 1-7 (2020).
7. M.A. Rezaee, O. Pajand, M.R. Nahaei, R. Mahdian, M. Aghazadeh, and M. Ghojazadeh. Prevalence of
Ambler class A β-lactamases and ampC expression in cephalosporin-resistant isolates of Acinetobacter
baumannii. Diagnostic Microbiology and Infectious Disease 76(3): 330-334 (2013).
8. A.A. El Shamy, Z. Zakaria, M.M. Tolba, N. Salah Eldin, A.T. Rabea, M.M. Tawfick, and H.A. Nasser. AmpC β-Lactamase Variable Expression in Common Multidrug-Resistant Nosocomial Bacterial Pathogens from a Tertiary Hospital in Cairo, Egypt. International Journal of Microbiology 2021(1): 6633888 (2021).
9. S. Santajit, and N. Indrawattana. Mechanisms of antimicrobial resistance in ESKAPE pathogens. BioMed Research International 2016(1): 2475067 (2016).
10. S. Oie, Y. Fukui, M. Yamamoto, Y. Masuda, and A. Kamiya. In vitro antimicrobial effects of aztreonam,
colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-β-lactamase producing Pseudomonas aeruginosa. BioMed Central Infectious Diseases 9: 1-5 (2009).
11. M. Chatterjee, C. Anju, L. Biswas, V.A. Kumar, C.G. Mohan, and R. Biswas. Antibiotic resistance in Pseudomonas aeruginosa and alternative therapeutic options. International Journal of Medical Microbiology 306(1): 48-58 (2016).
12. A. Sule, and O. Olusanya. In-vitro antibacterial activities of fluoroquinolones compared with common antimicrobial agents against clinical bacterial isolates from parts of south western Nigeria. Nigerian Quarterly Journal of Hospital Medicine 10(1): 18-21 (2000).
13. G.F. Gad, R.A. El-Domany, S. Zaki, and H.M. Ashour. Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: prevalence, antibiogram and resistance mechanisms. Journal of Antimicrobial Chemotherapy 60(5): 1010-1017 (2007).
14. C.L. Ventola. The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and Therapeutics
40(4): 277 (2015).
15. A. Bauer, W. Kirby, J.C. Sherris, and M. Turck. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45(4): 493-496 (1966).
16. V.C. Yadav, V.R. Kiran, M.K. Jaiswal, and K. Singh. A study of antibiotic sensitivity pattern of Pseudomonas aeruginosa isolated from a tertiary care hospital in South Chhattisgarh. International Journal of Medical Science and Public Health 6(3): 600-605 (2017).
17. R. Miladi, M.H. Zamanian, A. Janbakhsh, F. Mansouri, B. Sayad, M. Afsharian, S. Vaziri, Z.M. Afshar, M. Shirvani, M. Tarlan, and S. Khazaei. Antibiotic Resistance of Pseudomonas aeruginosa Strains in the Patients Admitted to Imam Reza Hospital in Kermanshah, Iran (2016-2018). Journal of Kermanshah University of Medical Sciences 24
(4): (2020).
18. S.A. Hasan, A.M. Najati, and K.S. Abass. Prevalence and antibiotic resistance of “Pseudomonas aeruginosa” isolated from clinical samples in Kirkuk City, Iraq. EurAsian Journal of Biosciences 14(1): 1821-5 (2020).
19. A.B. Mahmoud, W.A. Zahran, G.R. Hindawi, A.Z. Labib, and R. Galal. Prevalence of multidrug
resistant Pseudomonas aeruginosa in patients with nosocomial infections at a university hospital in Egypt, with special reference to typing methods. Journal of Virology and Microbiology 13: 165-59 (2013).
20. M.A. Khan, and A. Faiz. Antimicrobial resistance patterns of Pseudomonas aeruginosa in tertiary care hospitals of Makkah and Jeddah. Annals of Saudi Medicine 36(1): 23-28 (2016).
21. K. Pokharel, B.R. Dawadi, C.P. Bhatt, and S. Gupte. Prevalence of Pseudomonas aeruginosa and its
antibiotic sensitivity pattern. Journal of Nepal Health Research Council 17:109-113 (2019).
22. A. Mohamed, and F. Abdelhamid. Antibiotic susceptibility of Pseudomonas aeruginosa isolated
from different clinical sources. Zagazig Journal of Pharmaceutical Sciences 28(2): 10-17 (2020).
23. R. Jawad. Antibiotic susceptibility patterns of Pseudomonas aeruginosa isolated from clinical and hospital environmental samples in Nasiriyah, Iraq.
24. G. Feretzakis, E. Loupelis, A. Sakagianni, N. Skarmoutsou, S. Michelidou, A. Velentza, M. Martsoukou, K. Valakis, S. Petropoulou, and E. Koutalas. A 2-year single-centre audit on antibiotic resistance of Pseudomonas aeruginosa,
Acinetobacter baumannii and Klebsiella pneumoniae strains from an intensive care unit and other wards in a general public hospital in Greece. Antibiotics 8(2): 62 (2019).
25. G. Jombo, P. Jonah, and J. Ayeni. Multidrug resistant Pseudomonas aeruginosa in contemporary medical
practice: findings from urinary isolates at a Nigerian University Teaching Hospital. Nigerian Journal of
Physiological Sciences 23: 1-2(2008).
26. G. Shewatatek, T. Gizachew, B. Molalegne, and G. Terefe. Drug sensitivity of Pseudomonas aeruginosa
from wound infections in Jimma University Specialized Hospital, Ethiopia. Journal of Medical Sciences Res 3(2): 13-18 (2014).
27. K. Okon, P. Agukwe, W. Oladosu, S. Balogun, and A. Uba. Antibiotic resistance pattern of Pseudomonas
aeruginosa isolated from clinical specimens in a tertiary hospital in Northeastern Nigeria. Journal of Microbiology 8(2): 5-7 (2009).
28. E. Bouza, R. San Juan, P. Muñoz, A. Voss, and J. Kluytmans. Co‐operative Group of the European Study Group on Nosocomial Infections. A European perspective on nosocomial urinary tract infections I. Report on the microbiology workload, etiology and antimicrobial susceptibility (ESGNI‐003 study). European Study Group on Nosocomial Infections. Clinical Microbioogy and Infection 7(10): 523-531 (2001).
29. D.A. Shah, S. Wasim, and F.E. Abdullah. Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from urine samples of Urinary Tract Infections patients in Karachi, Pakistan. Pakistan Journal of Medical Sciences 31(2): 341 (2015).
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