Mild Alkaline Pretreatment on Sugarcane Bagasse: Effects of Pretreatment Time and Lime to Dry Bagasse Ratio
Mild Alkaline Pretreatment on Sugarcane Bagasse
Keywords:
Bagasse, Bioethanol, Enzymatic Hydrolysis, PretreatmentAbstract
Sugarcane waste is organic waste which has produced at about 8 × 106 tons annually in Indonesia. This lignocellulosic waste can be used as raw material for bioethanol manufacture through pretreatment, hydrolysis, and fermentation process. This study aims to evaluate system parameters of bagasse pretreatment using lime (Ca(OH)2) in a batch system and to obtain the yield (g/g) of glucose produced from pretreated bagasse via enzymatic hydrolysis. The variables used in this study were pretreatment time and lime ratio to bagasse in the pretreatment step. It was then followed by a hydrolysis step to produce glucose. The fermentation was conducted to investigate how much ethanol can be produced at the best glucose yield. The results showed that pretreatment time gave a stronger effect on the glucose yield compared to lime ratio to bagasse. Longer pretreatment time showed the increased glucose yield in the hydrolysis of pretreated bagasse. However, after 180 minutes of pretreatment, glucose yield was decreased. Meanwhile, a higher ratio of lime to bagasse in the pretreatment process reduced the glucose yield. The best glucose
yield (0.071 g/g) was obtained from the pretreatment process with a ratio of 0.5 g Ca(OH)2 g–1 bagasse for 180 min; pH 5.0 and the ethanol content was (5.704 ± 0.15) g L–1.
References
M.F. Ibrahim,., S.W.Kim, S.Abd-Azi. Advanced bioprocessing strategies for biobutanol production from biomass. Renewable and Sustainable Energy Reviews 91:1192-1204 (2018).
J.S. Kim, Y.Y. Lee and T.H. Kim. A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresource Technology 199: 42–48(2016).
T.Fajriutami, W. Fatriasari, R.P.B. Laksana and E. Hermiati. Pengaruh Pretreatment NaOH dan Hidrolisis Enzimatis pada Ampas Tebu [Effect of Pretreatment of NaOH and Enzymatic Hydrolysis on Sugarcane Bagasse]. LIPI, Jakarta, Indonesia, p. 18–27; p. 29−40 (2013).(in Bahasa Indonesia).
I. Kim, M.S.U. Rehman and J. Han. Enhanced glucose yield and structural characterization of corn stover by sodium carbonate pretreatment. Bioresource Technology152:316–320(2014).
X. Liu, S.M. Zicari, G. Liu, Y. Li and R. Zhang. Pretreatment of wheat straw with potassium hydroxide for increasing enzymatic and microbial degradability. BioresourceTechnology185:150–157(2015).
J.Domanski, S. Borowski, O.M. Mikolajczyk and P. Kubacki. Pretreatment of rye straw with aqueous ammonia for conversion to fermentable sugars as a potential substrates in biotechnological processes. Biomass and Bioenergy 91: 91–97 (2016).
S.Kim, and M.T. Holtzapple. Lime pretreatment and enzymatic hydrolysis of corn stover.BioresourceTechnology 96:1994–2006(2005).
M.Inggrid, C. Yonathan, andH. Djojosubroto. Pretreatment sekam padi dengan alkali peroksida dalam pembuatan bioetanol [Pretreatment of rice husk with alkali peroxide in bioethanol making]. Universitas Katolik Parahyangan-Research report. 1–6 (2011). (in BahasaIndonesia).
H.Muljana, T. Handoko, L. Meilianasari and G. Widhi. Pengaruh media sub- dan superkritik CO2 dalam proses hidrolisis secara enzymatic terhadap perolehan glukosa [Effect of CO2 media and supercritics in the hydrolysis process on approval]. Research Report-Engineering Science 2: 1–31 (2013). (in Bahasa Indonesia).
I.A.Kartika, M. Yani, D. Ariono, P.H. Evon and L. Rigal. Biodiesel production from jatrophas seed: solvent extraction and in situ trans-esterification in single step. Fuel106:111–117(2013).
J. Xu, J.J. Cheng, R.R. SharmaShivappa and J.C. Burns. Lime pretreatment of switchgrass at mild temperatures for ethanol production. Bioresource Technology 101: 2900–2903(2010).
L.J. Jonsson, and C. Martin. Pretreatment of lignocellulose: Formation of inhibitory by products and strategies for minimizing their effects. Bioresource Technology 199: 103–112 (2016).
S.C.Rabelo, S.C., N.A.A. Fonseca, R.R. Andrade, R.M. Filho and A.C. Costa. Ethanol production from enzymatic hydrolysis of sugarcane bagasse pretreated with lime and alkaline hydrogen peroxide. Biomass and Bioenergy 35: 2600–2607 (2011).
Y.C. Li, Z.X. Gou, Y. Zhang, Z.Y. Xia, Y.Q. Tang and K. Kida. Inhibitor tolerance of a recombinant flocculating industrial saccharomyces cerevisiae strain during glucose and xylose co-fermentation. Brazilian Journal of Microbiology 48: 1–10 (2017).
Downloads
Published
How to Cite
Issue
Section
License
Creative Commons Attribution (CC BY). Allows users to: copy the article and distribute; abstracts, create extracts, and other revised versions, adaptations or derivative works of or from an article (such as a translation); include in a collective work (such as an anthology); and text or data mine the article. These uses are permitted even for commercial purposes, provided the user: includes a link to the license; indicates if changes were made; gives appropriate credit to the author(s) (with a link to the formal publication through the relevant DOI); and does not represent the author(s) as endorsing the adaptation of the article or modify the article in such a way as to damage the authors' honor or reputation.