Alpha Mangosteen Effect on MDA Level and the Pancreatic Morphology Rattus norvegicus (Berkenhout, 1769) Induced by Alloxan

Alpha Mangosteen Effect on MDA Level and the Pancreatic Morphology

Authors

  • Devyana Dyah Wulandari Department of Medical Laboratory Technology, Faculty of Health, Nahdlatul Ulama Surabaya University, Jl. Jemursari, 51–57, Surabaya, East Java, 60237, Indonesia
  • Zumrotul Mufidah Department of Medical Laboratory Technology, Faculty of Health, Nahdlatul Ulama Surabaya University, Jl. Jemursari, 51–57, Surabaya, East Java, 60237, Indonesia
  • Taslim Ersam Department of Chemistry, Faculty of Science, Sepuluh Nopember Institute of Technology, Jl. Keputih, Sukolilo, Surabaya, East Java, 60111, Indonesia
  • Devyani Diah Wulansari Department of Pharmacy, Faculty of Pharmacy, University of Surabaya, Jl. Tenggilis Mejoyo No.12, Rungkut, Surabaya, East Java, 60293 Indonesia
  • Alfredo Vita Corpuz Department of Medical Laboratory Technology, University of Northern Philippines, Tamag, Vigan City, Ilocos Sur, 2700, Philippines

Keywords:

Beta cells, Diabetes mellitus, Garcinia mangostana L., Lipid peroxidation

Abstract

The alpha mangosteen compound is a single compound isolated from rind extract Garcinia mangostana Linn, known to have antidiabetic bioactivity. This research aimed to determine the effect of the α-mangosteen compound on Malondialdehyde (MDA) and rats pancreatic tissue induced with type 2 Diabetes mellitus. Twentyfour Wistar male rats were divided into four groups, each group consisting of six rats. The first group was set as a positive control, rats injected with a single dose of 150 mg kg–1 alloxan. The second group was the negative control, which means that no treatment. The third group was injected with a single dose of 150 mg kg–1 alloxan and α-mangosteen compound 10 mg kg–1, while the last group was injected with a single dose of 150 mg kg–1 alloxan and 10 mg kg–1 standard drug glibenclamide. All groups were monitored for 3 wk, and blood sugar levels were measured using a Glucose meter. Blood samples were taken for the measurement of MDA, and the pancreas organ was removed for histological study. This research showed that the difference of MDA level between α-mangosteen compound
treatment and glibenclamide was only 0.481 8. It means that the α-mangosteen compound can reduce MDA levels alike the standard drug glibenclamide capabilities. Islets of Langerhans on normal pancreatic mice (control (-)) looked filling full with the endocrine cells spread throughout the pancreas. While at the control group (+) there was a roomempty space (fewer the number of beta cells) in islets of Langerhans. On the treatment groups, those were giving α-mangosteen, and drug compounds standard (glibenclamide) showed improvement in Langerhans β cells compared with a treatment group of diabetic mice (control+). The administration of α-mangosteen compound 10 mg kg–1 BW able to decrease MDA level 1.204 7 nmol dl–1. While based on histological observations of the pancreas showed that the alpha mangosteen treatment group showed improvement of Langerhans β cells compared to the diabetic rat group (positive control)

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Published

2021-06-08

How to Cite

Wulandari, D. D., Mufidah, Z., Ersam, T. ., Wulansari, D. D. ., & Corpuz, A. V. . (2021). Alpha Mangosteen Effect on MDA Level and the Pancreatic Morphology Rattus norvegicus (Berkenhout, 1769) Induced by Alloxan: Alpha Mangosteen Effect on MDA Level and the Pancreatic Morphology. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 57(4), 15–21. Retrieved from https://ppaspk.org/index.php/PPAS-B/article/view/313

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