Effects of Plant Extracts on Bacterial Isolates from Infections of the Female Genital Tract
DOI:
https://doi.org/10.53560/PPASB(60-2)820Keywords:
Candida albicans, Bacterial species, Plant extracts, Infectious diseasesAbstract
Fifty samples of female genital secretions were collected from a group of women whose age ranges from 15 to 50 years old at Al-Batool Hospital for Maternity and Children. The isolates Escherichia coli, Staphylococcus aureus, Candida albicans, and Staphylococcus epidermidis were found in the fungal and bacterial cultures of the samples, with percentages of (45, 31, 13, 11), respectively. Positive samples were isolated at the highest rate of 87 percent for samples isolated from people aged 30 to 40. In the case of pregnancy, there is an increase in the infection rate with fungal and bacterial species, with a percentage of 60 % compared to women. The results of testing the effect of plant extracts (marjoram, chamomile, thyme, peat, and thuja) on isolated bacterial and fungal species showed inhibition diameters (20, 16, 15.5, 13, 10, 10) mm at concentrations (25 %, 50 %, 75 %, 100 %). We discovered that different concentrations of Marjoram, chamomile, thyme, peat, and thuja extracts (25 %, 50 %, 75 %, 100 %) were associated with increased inhibition rates of fungal and bacterial infection with different diameters (10, 12, 15, 16, 20 mm).
References
S. Das. Natural therapeutics for urinary tract infections-a review. Future Journal of pharmaceutical sciences, 6(1): 64 (2020). https://doi.org/10.1186/s43094-020-00086-2.
F. Azmodeh, S.H. Hajikhani,, and S. Alizadeh. Evaluation of the hydroalcoholic chamomile extract antifungal activity on Candida albicans-Invitro Study. Journal of Research in Dental Sciences 13:4 (2016).
M.A. Pequeno. Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Enterobacter Cloacae biofilms: in vitro study. RGORevista Gaúcha de Odontologia 66: 122-128 (2018).
A Azari, and A. Danesh. Antibacterial effect of Matricaria chamomilla alcoholic extract against drug-resistant isolates of Staphylococcus aureus and Pseudomonas aeruginosa. Infection Epidemiology and Microbiology 7:29-35 (2021).
K. Rajkowska, N. Paulina, K. Nowicka, and A.K. Styczyńska. Effect of clove and thyme essential oils on Candida biofilm formation and the oil distribution in yeast cells. Molecules 24:10 (2019)
S. Xiao, P. Cui, W. Shi, and Y. Zhang Identification of essential oils with activity against stationary phase Staphylococcus aureus. BMC complementary medicine and therapies 20: 99 (2020). https://doi.org/10.1186/s12906-020-02898-4.
B.J. Kullberg, and C. Maiken. Invasive candidiasis. New England Journal of Medicine 373:1445-1456. (2015)
A. Barbosa, D. Araújo, E. Ribeiro, M. Henriques. Candida albicans adaptation on simulated human body fluids under different pH. Microorganisms 8:511(2020).
G.L. Qiao, J. Ling, T. Wong, S.N. Yeung, and A. Iovieno. Candida Keratitis: Epidemiology, Management, and Clinical Outcomes. Cornea, 39:7 801-805. (2020).
G. Brunetti. Candida blood stream infections observed between 2011 and 2016 in a large Italian University Hospital: A time based retrospective analysis on epidemiology, biofilm production, antifungal agents consumption and drugsusceptibility. PLoS One 14:11 (2019)
D. Toledo, and L. Gaspar. Improved in vitro and in vivo Anti-Candida albicans Activity of Cymbopogon nardus Essential Oil by Its Incorporation into a Microemulsion System. International Journal of Nanomedicine 15:10481 (2020)
D.W. Denning. Global burden of recurrent vulvovaginal candidiasis: a systematic review. The Lancet infectious diseases 18:11 (2018)
A. Bitew. The prevalence, antibiotic resistance pattern, and associated factors of bacterial vaginosis among women of the reproductive age group from felege hiwot referral hospital, Ethiopia. Infection and Drug Resistance 14:2685 (2021)
E. Magliano. Gender and age-dependent etiology of community-acquired urinary tract infections. The scientific world journal 2012 (2012).
O.P. Aduloju, A.A. Akintayo, and T. Aduloju. Prevalence of bacterial vaginosis in pregnancy in a tertiary health institution, south western Nigeria. The Pan African Medical Journal 33 (2019).
M.J. Mucci. Prevalence of Candida albicans, Candida dubliniensis and Candida africana in pregnant women suffering from vulvovaginal candidiasis in Argentina. Revista iberoamericana de micologia 34:2 (2017)
G. Nahed.m Phenotypic and genotypic characterization of extended-spectrum betalactamases produced by Escherichia coli colonizing pregnant women. Infectious Diseases in Obstetrics and Gynecology 2020 (2020).
A. Tsega, and M. Feleke. Prevalence, risk factors and antifungal susceptibility pattern of Candida species among pregnant women at Debre Markos Referral Hospital, Northwest Ethiopia. BMC pregnancy and childbirth 19:1 (2019)
D. N. Anh, D. N. Hung, T. V. Tien, and V. N. Dinh. Prevalence, species distribution and antifungal susceptibility of Candida albicans causing vaginal discharge among symptomatic non-pregnant women of reproductive age at a tertiary care hospital, Vietnam. BMC infectious diseases 21.1 (2021): 1-10.
M. Haque. Health care-associated infections–an overview. Infection and drug resistance 11:2321 (2018)
J. Lin. A prospective cohort study of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus carriage in neonates: the role of maternal carriage and phenotypic and molecular characteristics. Infection and drug resistance 11:555 (2018)
Y. Moradi. A systematic review and meta-analysis on the prevalence of Escherichia coli and extendedspectrum β-lactamase producing Escherichia coli in pregnant women. Archives of Gynecology and Obstetrics 303:2 (2021)
L. Surgers. Escherichia coli bacteraemia in pregnant women is life-threatening for foetuses. Clinical Microbiology and Infection 20:12 1035-1041 (2014)
D. Danino. Mother-to-child transmission of extended-spectrum-beta-lactamase-producing Enterobacteriaceae. Journal of Hospital Infection 100: 40-46 (2018)
P.L. Stanojevic. Chemical composition, antioxidant and antimicrobial activity of chamomile flowers essential oil (Matricaria chamomilla L.). Journal of essential oil bearing plants 19: 2017-2028 (2016)
H. M. Alkuraishy. In vitro assessment of the antibacterial activity of Matricaria chamomile alcoholic extract against pathogenic bacterial strains. British Microbiology Research Journal 7:55-61 (2015)
S. Sadat, A.A. Azari, and M. Mazandarani. Evaluation of antibacterial activity of ethanolic extract of Matricaria chamomilla, Malva sylvestris and Capsella bursa-pastoris against methicillinresistant Staphylococcus aureus. Journal of Medical Microbiology and Infectious Diseases 8: 127-131 (2020)
N.A. Alshaikh, and K. Perveen. Susceptibility of Fluconazole-Resistant Candida albicans to Thyme Essential Oil. Microorganisms 9: 2454 (2021).
F. Al-Shoaibi, and S. Benedicts. Inhibitory effect of Garlic oil, Clove oil and Thyme oil on Micrococcus Luteus and Staphylococcus epidermidis. Journal of Student Research 10:3 (2021)
B. Lakhrissi. Antifungal activity of essential oil of oregano (Origanum vulgare), marjoram (Origanum majorana) and synergy of two essential oils against Candida albicans. International Journal of Research Studies in Science, Engineering and Technology:14-17 (2016).
E. Bona, Sensitivity of Candida albicans to essential oils: are they an alternative to antifungal agents? Journal of applied microbiology 121: 1530-1545 (2016)
M. Sienkiewicz, M. Wasiela, and A. Głowacka. The antibacterial activity of oregano essential oil (Origanum heracleoticum L.) against clinical strains of Escherichia coli and Pseudomonas aeruginosa. Medycyna Doswiadczalna i Mikrobiologia 64: 297-307 (2012)
M.G. Cattelan. Combined effects of oregano essential oil and salt on the growth of Escherichia coli in salad dressing. Food microbiology 73: 305-310 (2018)
F. Gomes. Satureja montana L. and Origanum majorana L. decoctions: Antimicrobial activity, mode of action and phenolic characterization. Antibiotics 9(6): 294 (2020)
D.S. Rodrigues, and J. Bezerra. Effects of oregano essential oil and carvacrol on biofilms of Staphylococcus aureus from food contact surfaces. Food Control 73;1237-1246 (2017)
D. Mahdiyah. Screening of Indonesian peat soil bacteria producing antimicrobial compounds. Saudi Journal of Biological Sciences 27: 2604-2611 (2020) https://doi.org/10.1016/j.sjbs.2020.05.033.
A.A. Hammadi, A. Jasim, A. Abdullah, and H. A. Hadeel. Study of inhibition efficiency of peat against burn infection bacteria Scientific Journal of Medical Research Vol 1, Issue 2, pp 78 -81 (2017).
A.N. Kapse, and C.J. Chandekar. Antimicrobial activity of leaves of thuja compacta against pathogenic organisms compared with control drugs. European Journal of Biomedical 7: 197-201 (2020)
B.J. Mohammed. Inhibition Activity of Thuja occidentalis Extract Against Staphylococcus aureus and Candida albicans Isolated form cases of Skin Infections. Iraq journal of market research and consumer protection 4:1 (2012)
N. Jain, S. Meenakshi. Chemical Characterization and Antidermatophytic Activity of Thuja orientalis Collected from Jaipur District, Rajasthan. Current Trends in Biotechnology & Pharmacy 11:3 (2017).
E. Jawetz, J.A. Melinck, E.A Adelbergc, G. Fand, and S. S. Morse. Medical Microbiology. 24thed. Mc. Graw Hill (2007).
C. Anesini, and C. Perez. Screening of plants used in Argentine folk medicine for antimicrobial activity. Journal of ethnopharmacology 39.2 119-12 (1993)
L.M. Prescott, J.P. Harley, and D.A. Klein. “Microbiology, Wm. C. Brown Communication.” Inc., 2nd. Edition, USA 912 (1993).
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