Validation of A Novel Atomic Absorption Spectrophotometric Technique in Determination of Ciprofloxacin Using High Pressure Liquid Chromatography as a Reference Method

Validation of a Novel AAS Technique


  • Amina Habib Faculty of Pharmacy University of Lahore, Lahore
  • Farooq Saleem Faculty of Pharmacy University of Lahore, Lahore
  • Saeed-Ul- Hassan Faculty of Pharmacy University of Lahore, Lahore
  • Habib Amjad Institute of Chemistry, University of the Punjab, Lahore


Atomic Absorption Spectrophotometry (AAS), High Pressure Liquid Chromatography (HPLC), Methyl Isobutyl Ketone (MIBK), Ferric Fluoride (FeF3), Ammonium Chloride (NH4Cl), Sodium Fluoride (NaF), Ciprofloxacin (CIP), Reverse Phase Column 18 (RPC18)


Same quantities of ciprofloxacin (0.2, 0.4, 0.6, 0.8 and 1.00 μg/liter) were determined by using atomic absorption Spectrophotometric (AAS) and high pressure liquid chromatography (HPLC) techniques. AAS technique was introduced as the test method where HPLC method was applied as the reference method as to validate the results of test method. In HPLC method reverse phase C18 column (RPC18 column) was used where mixture of acetonitrile: Phosphate buffer (35:65) was employed as mobile phase. In AAS method 284 nm wavelength revealed the maximum absorption and therefore further all the measurements were recorded using this wavelength. In AAS test technique, at first step ciprofloxacin was fused with sodium biphenyl. Then sodium fluoride (NaF) formed was reacted with FeCl3 to give iron fluoride (FeF3). The extra amount of FeCl3 was removed by reacting it with ammonium thiocyanate. Ferric thiocyanate (FeSCN) was selectively dissolved in Methyl Isobutyl Ketone (MIBK) and separated by filtration. NH4Cl and ferric thiocyanate were held back. Amount of Fluorine (F) was determined in terms of Fe and using this amount, quantity of ciprofloxacin was calculated.


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How to Cite

Habib, A. ., Saleem, F. ., Hassan, S.-U.-., & Amjad, H. . (2021). Validation of A Novel Atomic Absorption Spectrophotometric Technique in Determination of Ciprofloxacin Using High Pressure Liquid Chromatography as a Reference Method: Validation of a Novel AAS Technique. Proceedings of the Pakistan Academy of Sciences: A. Physical and Computational Sciences, 56(1), 53–57. Retrieved from