Kinetics and Thermodynamic Study of Cellulase Embedded Metal Organic Frameworks

Authors

  • Kainat Zahra Department of Biological Sciences, Superior University, Lahore, Punjab, Pakistan
  • Hina Zain Department of Biological Sciences, Superior University, Lahore, Punjab, Pakistan
  • Nazia Kanwal Department of Biological Sciences, Superior University, Lahore, Punjab, Pakistan
  • Jamal Ahamed Department of Biological Sciences, Superior University, Lahore, Punjab, Pakistan
  • Aysha Bukhari Department of Chemistry, Minhaj University, Lahore, Pakistan
  • Ammara Nazir Department of Chemistry, Minhaj University, Lahore, Pakistan
  • Athar Hussain Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Karachi, Sindh, Pakistan

DOI:

https://doi.org/10.53560/PPASA(61-3)680

Keywords:

Cellulase, Immobilization, Metal-organic Framework, Kinetics, De-novo Synthesis

Abstract

Cellulase is an important enzyme used for many purposes in different industrial sectors. Even though cellulase has so many applications, it easily denatures with a little change in pH and temperature, which causes low stability, usability, and activity. To enhance its activity and stability, immobilization of porous materials is the best way to enhance its activity, stability, and life span. For immobilization, a Metal-organic framework (MOF) is considered as a potential candidate. Cellulase@Zn- benzene 1-4 di-carboxylic acid (BDC) by hydrothermal method and Zn-cellulase-benzene 1-4 dicarboxylic acid (BDC) by de novo approach were prepared, and their activities were analyzed. Zn-cellulase-benzene 1-4 dicarboxylic acid (BDC) produced by the de novo approach, shows higher activity, stability, catalytic performance, and life span than the free enzyme, and cellulase@Zn- benzene 1-4 di-carboxylic acid (BDC) produced by the hydrothermal method.

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Published

2024-09-23

How to Cite

Kainat Zahra, Hina Zain, Nazia Kanwal, Jamal Ahamed, Aysha Bukhari, Ammara Nazir, & Athar Hussain. (2024). Kinetics and Thermodynamic Study of Cellulase Embedded Metal Organic Frameworks. Proceedings of the Pakistan Academy of Sciences: A. Physical and Computational Sciences, 61(3). https://doi.org/10.53560/PPASA(61-3)680

Issue

Section

Research Articles