Optimization of Indole-butyric Acid Competency on Root Elongation of Arecaceae through Micro-propagation

Indole-butyric Acid Competency on Root Elongation of Arecaceae

Authors

  • Asad Ali Khaskheli Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, Tandojam-70060, Pakistan , Institute of Cell Biology, School of Life Sciences, Lanzhou University, Cheng Guan, Lanzhou-730000, Gansu, P.R. China
  • Muhammad Ibrahim Khaskheli Department of Plant Protection, Faculty of Crop Protection, Sindh Agriculture University Tandojam- 70060, Pakistan
  • Muhammad Rafique Rind Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, Tandojam-70060, Pakistan
  • Asad Ali Khaskheli Department of Animal Nutrition, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, Pakistan
  • Li Zhang Institute of Cell Biology, School of Life Sciences, Lanzhou University, Cheng Guan, Lanzhou-730000, Gansu, P.R. China
  • Khadim Ali Panhwar Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, Tandojam-70060, Pakistan

Keywords:

Indole-butyric Acid, Micro-propagation, MS-Basal Medium, Offshoots, Sterilization

Abstract

In the present study optimization of Indole-butyric Acid (IBA) competency on the root elongation was evaluated. The regenerated shoots were separated and shifted to a new media (MS-Basal) containing various concentrations of (IBA) 0.0, 0.5, 1.0, 2.0, 3.0, and 4.0 mg/L and the observations based on the survival rate (%),initiation of roots at a different interval, encountering the total number of roots per shoot and roots length (cm) was noticed. The plants regenerated on (control) MS basal medium where zero concentration of IBA was used, revealed a relatively (P < 0.05) lower rate of survival (72.50%) as well as no roots observed till the end of the experiment. While MS-Basal medium supplemented with IBA (2.0mg/L) took minimum time to initiate the roots (16.80 days). The highest number of roots (4.48) appeared in the media containing 3mg/L IBA concentration, whilst 0.5 and 4.0mg/L
IBA demonstrated significantly (P > 0.05) similar response i.e. 1.83 and 1.70 number of roots respectively, that consequently revealed relatively less number of roots as compared to other concentrations of the media. The MS-Basal media supplemented with 2.0 and 4.0mg/L IBA showed relatively (P < 0.05) longer length of roots (2.45 and 2.22 cm) compared to that of MS-Basal media supplemented with 0.5 and 1.0mg/L IBA (1.46 and 1.60 cm, respectively).Besides these things, MS-Basal media supplemented with 3.0 mg/L IBA showed a higher length of roots than the other media.

References

J. M, Al-Khayri, and A.M. Al-Bahrany. Silver nitrate and 2-isopentyl adenine promote somatic embryogenesis in date palm (Phoenix dactylifera L.). Scientia Horticulturae 89: 291-298 (2001).

Al Khateeb A.A. The problems facing the use of tissue culture technique in date palm (Phoenix dactylifera L.). Sci. J. King Faisal Univ. 9: 85-104(2008).

O. Abass, M. Jasim, O.A. Razzak and N.O.H.Al-Meer. Effect of sucrose, light intensity and agriculture medium on acclimation of date palm (Phoenix dactylifera L. cv. barhee) propagated in vitro. Rmtla Plkhan Thahablo Prsbla Magazine 7(2):17-30 (2008).

J.M. Al-Khayri. In vitro germination of somatic embryos in date palm: Effect of auxin concentration and strength of MS salts. Current Science 84:5(2003).

A.M. Jasim, A.M.W. Al-Mayahi, A.H.M Attaha Propagation of four rare cultivars of date palm (Phoenix dactylifera L.) by tissue culture techniques.Basrah Journal for Date Palm Research, 8 (1): 72–99. (2009).

S.A. Bekheet, and M.M. Saker. In vitro propagation of Egyptian date palm: direct and indirect shoot proliferation from shoot-tip explants of Phoenix dactylifera L. cv. zaghlool. J. Food, Agri. and Env.Pp. 150-155 (2007).

J. Aslam, and S.A. Khan. Invitro Micropropagation of ‘Khalas’ date palm (Phoenix dactylifera L.), an important fruit plant. J. Fruit and OrnamentalPlant Research, 17(1): 15-27 (2009).

J.A.T. Amaral, A.B. Rena, A.T. Cordeiro, E.R. Schmildt. Effects of aluminum, nitrate and ammonium on the growth, potassium content and composition of amino acids in Stylosanthes. Idesia,31 (2): 61–68 (2013).

M.F. Gabr, and M.M. Abd-Alla. Micropropagation of Phoenix dactylifera L. var karama. New York Sci. J. 3(12):64-69 (2010).

I. Hussain, H. Rashid and A. Quraishi. In vitro multiplication of date palm: In Second International Conf on Date Palm: 24-27th March, Al-Alin, U.A.E.Industrial Research, Pp. 303-305 (2001).

T. Murashige, and F.A. Skoog. . A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol, 15: 473-479 (1962).

G.R. Rout, S. Samantaray and P. Das. Biotechnology of the Banana: A Review of Recent Progress. Plant Biology 2: 512-524 (2011).

N. Roux, J. Dolezel, R. Swennen and F.J. ZapataArias. Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp.Plant Cell, Tiss. Org. Cult. 66: 189-197 (2001).

I. Hussain, H. Rashid and A. Quraishi. In vitro multiplication of date palm: In Second International Conf on Date Palm: 24-27th March, Al-Alin, U.A.E.Industrial Research, Pp. 303-305 (2001).

T. Murashige, and F.A. Skoog. A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol, 15: 473-479 (1962).

S.C. Bhargava , S.N. Saxena and R. Sharma. In vitro multiplication of Phoenix dactylifera L. J. Biocehm. and Biotech., 12(1) 43-47 (2003).

M. Biroscikova, K. Spisakova, S. Liptak and V. Pichler. Micropropagation of mature wychelm (Ulmus glabra Huds.). Plant Cell Rep.,

:640-644 (2004).

S.F. El-Sharabasy, and Z.E. Zaid. Effect of plant growth regulators on the development of ex vitro date palm (Phoenix dactylifera L.) Barhi cv. Plantlets. Arab J. Biotech., 13(1):35-46 (2010).

A.A. El-Soaud, N.R. Sherbeny and E.S. Bakr. Organogenesis in female inflorescence of date palm (Phoenix dactylifera L. cv. Zaghloul). The Second International Conference on Date palm. Horticulture Institute, Agriculture Research Center, Egypt, Pp. 139-163 (2004).

Ali, B., Hasans. A., Hayats, Q., Yadv, S., Fariduddin, Q., Ahmed, A., 2008. A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean.

P. Eshraghi, R. Zarghami and M. Mirabdulbaghi. Somatic embryogenesis in two Iranian date palm cultivars. African J. Biotech., 4

(11): 1309-1312 (2005).

M.F. Gabr and B. Tisserat. Propagating palms in vitro with special emphasis on the date palm (Phoenix dactylifera L.). Scientia Horticulturae, 25(3): 255-262 (2003).

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Published

2021-06-02

How to Cite

Khaskheli, A. A., Khaskheli, M. I. ., Rind, M. R. ., Khaskheli, A. A. ., Zhang, L. ., & Panhwar, . K. A. (2021). Optimization of Indole-butyric Acid Competency on Root Elongation of Arecaceae through Micro-propagation: Indole-butyric Acid Competency on Root Elongation of Arecaceae . Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 57(4), 23–32. Retrieved from http://ppaspk.org/index.php/PPAS-B/article/view/305

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