Organic Acid and Nutrient Composition of Lactic Acid Bacteria Inoculated Total Mixed Ration Silage under Tropical Condition

Organic Acids and Nutrients Composition of Lactic Acid Bacteria

Authors

  • Ahmad Wahyudi Faculty of Agricultural and Animal Science, Muhammadiyah Malang University, Malang 65144, Indonesia
  • Dicky Pamungkas Institute of Beef Cattle Research, Jl. Pahlawan, Grati, Pasuruan 67184, Indonesia
  • Roy Hendroko Setyobudi Ma Chung Research Center for Photosynthetic Pigments, Vila Puncak Tidar N-01, Malang 65151, Indonesia , Indonesia Association of Bioenergy Scientist and Technologist, BPPT Building II, 22nd Floor, Jl. MH. Thamrin No. 8 Jakarta 10340
  • Listriari Hendraningsih Faculty of Agricultural and Animal Science, Muhammadiyah Malang University, Malang 65144, Indonesia
  • Zane Vincēviča-Gaile Department of Environmental Science, University of Latvia, Raina Blvd. 19, Riga, LV-1586, Latvia, European Union

Keywords:

Lactic acid bacteria, nutrient composition, organic acid, silage, total mixed ration

Abstract

Organic acids and nutrients composition of total mixed ration (TMR) silage inoculated with local Lactic acid bacteria (LAB) derived from paddy rice was studied. This study was conducted to prepare TMR silage in tropical climate. LAB was isolated from local paddy rice that was planted around Muhammadiyah University of Malang, East Java, Indonesia. The local LAB ability was
compared with commercial strain L. plantarum FCC 123 for organic acids and nutrients composition on TMR silage fermentation. The study was designed by completely randomized with five treatments (T0: TMR silage without LAB, T1: TMR silage + L. plantarum FCC 123, T2: TMR silage + local LAB Ciherang 1, T3: TMR silage + local LAB Membramo 1, T4: TMR silage + local LAB Rajalele 1), each treatment was replicated four times. This study showed the inoculation of local LAB in TMR silages preparation significantly (P ≤ 0.01) increased lactic acids production and tended to reduce acetic acid and pH value compare to control. There were not different all treatment and control on nutrients composition. It could be concluded that the local LAB inoculant had ability similar to L.plantarum FCC 123 on improving quality of TMR silage.

References

Reiber, C., R. Schultze-Kraft, M. Peters, P. Lentes,& V. Hoffmann. Promotion and adoption of silage technologies in drought-constrained areas of Honduras. Tropical Grasslands 44: 231–245(2010).

Nishino, N., H. Harada, & E. Sakaguchi. Evaluation of fermentation and aerobic stability of wet brewers’ grains ensiled alone or in

combination with various feeds as a total mixed ration. Journal of Science Food Agriculture 83: 557–563 (2003).

Ohmomo, S., O. Tanaka, H.K. Kitamoto, & Y. Cai. Silage and microbial performance: Old story but new problems. Japan Agricultural Research Quartely 36 (2): 59–71 (2002).

Khota, W., S. Pholsen, D. Higgs, & Y. Cai. Natural Lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant. Journal of Dairy Science 99: 1–14 (2016).

Pholsen, S., W. Khota, H. Pang, D. Higgs, & Y. Cai. Characterization and application of lactic acid bacteria for tropical silage preparation. Animal Sciences Journal 87 (10): 1202–1211 (2016)

Cai, Y., Y. Benno, M. Ogawa, S. Ohmomo, S. Kumai, & T. Nakase. Influent of Lactobacillus spp. from an inoculant and of Weisella and Leuconostoc spp. from forage crops on silage fermentation. Applied and Environmental Microbiology 64:2982−2987 (1998).

Cai, Y., Y. Benno, M. Ogawa, & S. Kumai. Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage. Journal of Dairy Science 82: 520−526 (1999).

Cai, Y., S. Kumai, M. Ogawa, Y. Benno, & T. Nakase. Characterization and identification of Pediococcus spp isolated from forage crops and their aplication for silage preparation. Applied and Environmental Microbiology 65: 2901−2906 (1999).

Wildan, F. Penetapan kadar asam laktat dalam silase dan cairan rumen dengan gas kromatografi [Determination of lactic acid content on silage and rumen fluid by gas chromatography]. Prosiding Temu Teknis Nasional Tenaga Fungsional Pertanian. Balai Penelitian Ternak, Ciawi-Bogor (2005). [in Bahasa Indonesia].

William, H., & W.L. George. Official Method of Analysis. 18th edn. Association of Official Analytical Chemists, Gaithersburg, Maryland,

USA. p. 2408−2417 (2005).

Filya, I., G. Ashbell, Y. Hen, & Z.G. Weinberg. The effect of bacterial inoculants on the fermentation and aerobic stability of whole crop

wheat silage. Animal Feed Science and Technology 88: 39−46 (2000).

Muck, R.E., I. Filya, & F.E. Contreras-Govea. Inoculant effects on alfalfa silage: In vitro gas and volatile fatty acid production. Journal of Dairy Science 90: 5115–5125 (2007).

Mc Donald, P., A.R. Henderson, & S.J.E. Heron. The Biochemistry of Silage. Chalcombe Publication, Marlow (1991).

Driehuis, F., S.J.W.H. Oude Elferink, & P.G. Van Wikselaar. Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria. Grass and Forage Science 56: 330–343 (2001).

Nkosi, B.D., R. Meeske, D. Palic, T. Langa, K-J. Leeuw, & I.B. Groenewald. Effects of ensiling whole crop maize with bacterial inoculants on the fermentation, aerobic stability, and growth performance of lambs. Animal Feed Science and Technology 154: 193−203 (2009).

Hu, X., W. Hao, H. Wang, T. Ning, M. Zheng, & C. Xu. Fermentation characteristics and lactic acid bacteria succession of total mixed ration silages formulated with peach pomace. Asian Australasian Journal Animal Sciences 28(4): 502-510 (2015).

Chen, L., G. Guo, X. Yuan, M Shimojo, C. Yu, & T Shao. Effect of applying molasses and propionic acid on fermentation quality and aerobic stability of total mixed ration silage prepared with wholeplant corn in Tibet. Asian Australasian Journal Animal Sciences 27(3): 349356 (2014)

Krooneman, J., F. Faber, A.C. Alderkamp, S.J. Elferink, F. Driehuis, I. Cleenwerck, et. al.Lactobacillus Diolivorans sp. nov., a 1, 2-

propanediol- degrading bacterium isolated from aerobically stable maize silage. International Journal of Systematic and Evolutionary

Microbiology 52(2): 639−646 (2002).

Huisden, C.M., A.T. Adesogan, S.C. Kim, & T. Ososanya. Effect of applying molasses or inoculants containing Homofermentative or

Heterofermentative bacteria at two rates on the fermentation and aerobic stability of corn silage. Journal of Dairy Science 92: 690–697 (2009).

Mari, L.J., R.J. Schmidt, L.G. Nussio, C.M. Hallada, & L. Kung. Short communication: An evaluation of the effectiveness of Lactobacillus

buchneri 40788 to alter fermentation and improve the aerobic stability of corn silage in farm silos. Journal of Dairy Science 92: 1174–1176 (2009).

Downloads

Published

2017-03-18

How to Cite

Wahyudi, A. ., Pamungkas, D. ., Setyobudi, R. H. ., Hendraningsih, L. ., & Vincēviča-Gaile, Z. . (2017). Organic Acid and Nutrient Composition of Lactic Acid Bacteria Inoculated Total Mixed Ration Silage under Tropical Condition: Organic Acids and Nutrients Composition of Lactic Acid Bacteria. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 54(1), 41–45. Retrieved from http://ppaspk.org/index.php/PPAS-B/article/view/374

Issue

Vol. 54 No. 1 (2017)

Section

Research Articles

License

Creative Commons Attribution (CC BY). Allows users to: copy the article and distribute; abstracts, create extracts, and other revised versions, adaptations or derivative works of or from an article (such as a translation); include in a collective work (such as an anthology); and text or data mine the article. These uses are permitted even for commercial purposes, provided the user: includes a link to the license; indicates if changes were made; gives appropriate credit to the author(s) (with a link to the formal publication through the relevant DOI); and does not represent the author(s) as endorsing the adaptation of the article or modify the article in such a way as to damage the authors' honor or reputation.