Antibacterial Activities and Chemical Characterization of the Secondary Metabolites of Aspergillus terreus
Antibacterial Activities of the Secondary Metabolites of Aspergillus terreus
DOI:
https://doi.org/10.53560/PPASB(60-2)786Abstract
The present study aims to assess the biological impact of secondary metabolites isolated from Aspergillus terreus that have been isolated from the soil, the fungus was grown on a fermentation medium to produce secondary metabolism, and the fungal extract extracted from the secondary metabolite was purified and chemically characterized. Antimicrobial activities of bioactive compounds extracted from the secondary metabolite of Aspergillus terreus isolate were tested against five types of human pathogenic bacteria, Escherichia coli, Klebsiella spp., Staphylococcus aureus, Pseudomonas spp., and Proteus spp. The cytotoxicity was tested against a human blood solution. purification and chemical identification were carried out on a crude extract of A. terreus using TLC, GC–MS, and NMR data (1H proton and 13C carbon) analysis. The A. terreus secondary metabolite extract was effective against all isolated bacterial strains. The biocompatibility test showed no cytotoxic effect against a human blood solution used in different concentrations. One fraction was purified and identified as a novel compound: 2-(4-hydroxyphenyl) tetrahydro-3,4- furan diol. The results from a GC–MS analysis showed 18 peaks of the ethyl acetate extract of A. terreus metabolites, and the major compounds were bis(2-ethylhexyl) phthalate (47.60 %), n-hexadecanoic acid (16.41 %) and dodecamethylcyclohexasiloxane (9.79 %). According to the outcomes of this study, A. terreus can produce secondary metabolites that show potent activity against human pathogenic bacteria and are dependent sources for new secondary metabolites.
References
K. Jawaid, M. Shafique, A. Versiani, H. Muhammed, S.A. Naz, and N. Jabeen. Antimicrobial potential of newly isolated Aspergillus terreus MK-1: An approach towards new antibiotics. The Journal of the Pakistan Medical Association 69(1): 18-23(2019).
B. Amina, G. Sana, J. Atef, D. Laid, and K. Noreddin. Antibacterial activity of Aspergillus isolated from different Algerian ecosystem. African Journal of Biotechnology 16(32): 1699-1704(2017).
P. Phainnphoug, C. Rukachaisirikul, C. Srimaroeng, A. Duangja, and J. ayaroj. Analogous from the soil-derived fungus Aspergillus sclerotiorum PsuRSPG178. Natural Products 79: 1500-150 (2016).
X. Zhang, Z. Li, and J. Gao. Chemistry, and biology of secondary metabolites from Aspergillus genus. The Natural Product Journal 8(4): 275–304( 2018).
D. Chun-Me, L. Shi-Xin, H. Cai-Huan, P. Ji-Yan, and L. Yong-Cheng . Secondary metabolites of Mangrove endophytic fungus Aspergillus terreus No.GX7-3B from the South China sea. Marine Drugs 11: 2616-2624(2013).
Y. Gung, H. Bao, B. Ya.,W. Qiang, Z. Xiao, L. Na, Q. Xue, and X. Li. Secondary metabolites of the fungus Aspergillus terreus. Chemistry of Natural Compounds 54(2): 415-418 ( 2018).
X. Lan-Lan, C. Fei, T. Sha-Sha, and Z. Hua-Jie. Investigations of Fungal Secondary Metabolites with Potential Anticancer Activity. Chemistry of Natural Compounds 53, 1212(2017).
M. Cadelis, A. Grey, S. Van de Pas, S. Geese, B. Weir, B. Copp, and S. Wiles. Terrien, a metabolite made by Aspergillus terreus, has activity against Cryptococcus neoformans. PeerJ 10: e14239(2022).
T. Ng, R. Cheung, J. Wong, A. Bekhit, and A.E. Bekhit. Antibacterial products of marine organisms. Applied Microbiology and Biotechnology 99(10): 4145-4173(2015).
C.J. Murray, K.S. Ikuta, F. Sharara, L. Swetschinski, G.R. Aguilar, A. Gray, C. Han, C. Bisignano, P. Rao, E. Wool, and S.C. Johnson. Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis Lancet 399(10325): 629–655.(2022).
F. Yakop, H. Taha, and P. Shivanand. Isolation of fungi from various habitats and their possible bioremediation. Current Science 116(5): 733-740(2019).
K.B. Raper, and D. I. Fennell. The genus Aspergillus. Williams and Wilkins Co., Baltimore, Md. 686 p. (1965).
N. Zainuddin, S.A. Alias, C.W. Lee, R.E bel , N. Othman, M.R. Muktar, and K. Awang. Antimicrobial activites of marine fungi from Malaysia. Botanica Marina 53: 507-513(2010).
L.A. Al-Timimi. Antibacterial and Anticancer Activities of Fenugreek Seed Extract. Asian Pacific Journal of Cancer Preventation 20 (12): 3771-3776(2019).
J.M. Miller, J. M. Binnicker, S. Campbell, K.C. Carroll, and K.C. Chapin et al. Laboratory Diagnosis of Infectious Disease. Clinical Infectious Diseases 67(6): 1-94 (2018).
Y.T. Wang, Y.R. Xue, and C.H. Liu. A brief review of bioactive metabolites derived from deep-sea fungi. Marine Drugs 13(8): 4594-4616 ( 2015).
W.t. Li, D. Luo, J.N. Huang, W. Ll, F.G. Zhang, T. Xi, and J.M. Liao, Y.Y. Lu. Antibacterial constituents from Antarctic fungus, Aspergillus sydowii SP-1. Natural Product Research 32(6): 662-667(2018).
H. Xian-guo, and M. Urasella. Antifungal compound from Solanum nigrum. Journal of Ethnopharmacology 43: 173-177(1994).
B. Kim, J. Park, H. Choi, S.S. Yoon, and W. Kim. Terrein is an inhibitor of quorum sensing and c-diGMP in Pseudomonas aeruginosa: a connection between quorum sensing and c-di-GMP. Scientific Reports 8(1): 8617(2018).
A. Bracarense, and J.T. Takahashi. Modulation of antimicrobial metabolites production by the fungus Aspergillus parasiticus. Brazilian Journal of Microbiology 45(1): 313-321(2014).
M. Femenía-Ríos, C.M. García-Pajón, R. HernándezGalán, A.J. Macías-Sánchez, and I.G. Collado. Synthesis and free radical scavenging activity of a novel metabolite from the fungus Colletotrichum gloeosporioides. Bioorganic & Medicinal Chemistry
Letters 16(22): 5836-5839(2006).
G. Mancilla, M. Femenía-Ríos, M. Grande, R. Hernández-Galán, A.J. Macías-Sánchez, and I.G. Collado. Enantioselective, chemoenzymatic synthesis, and absolute configuration of the antioxidant (−)gloeosporiol, Tetrahedron 66( 40): 8068-8075(2010).
Z.N. Al-Laith, L.A. Naser, and A.D. Al- Hilfi. Determination The Effect Of Helicobacter Pylori Toxin On Normal And Cancer Cells Lines. Turkish Journal of Physiotherapy and Rehabilitation 32(3) (2021).
F.N. Jafer, and L.A. Naser. The Biological Activity Of Aqueous And Methanolic Extracts Of Juglans Regia On Yeasts And Pathologic Bacteria. Plant Archives 20: 2405-2410(2020).
L.A. Al-Timimi, and N.A. Al-Tameemi. Role Of Carob Seed In Characterization Of MDR Bacteria In Diabetic Foot Ulcer. Biochemical and Cellular Archives 20: 927-934(2020).
G. Molinari. Natural products in drug discovery, present status and perspectives. Pharmaceutical Biotechnology 655: 13-27(2009).
S. Kandasamy, S. Sahu, and K. Kandasamy. In Silico Studies on Fungal Metabolite against Skin Cancer Protein (4,5-Diarylisoxazole HSP90 Chaperone). International Scholarly Research Notices 626214-5(2012).
S. Rajalakshmi, and N. Mahesh. Production and Characterization of Bioactive Metabolites Isolated from Aspergillus terreus in Rhizosphere Soil of Medicinal Plants. International Journal of Current Microbiology and Applied Sciences 3: 784-798(2014) .
L. Huang, X. Zhu, S. Zhou, Z. Cheng, K. Shi, C. Zhang, and H. Shao. Phthalic Acid Esters: Natural Sources and Biological Activities Toxins 13(7): 495(2021).
M.J. Sohn, S.J. Yoo, D.B. Oh, O.K. Won, S.Y. Lee, and A.A. Sibirny . Novel Cysteine-Centered Sulfur Metabolic Pathway in the Thermotolerant Methylotrophic Yeast Hansenula polymorpha 9(6): e100725(2014).
K. Jung, M. Miyagawa, A. Matsuda, Y. Amagai, K. Oida, Y. Okamoto, M. Takai, S. Nishikawa, H. Jang, S. Ishizaka, G. Ahn, A. Tanaka, and H. Matsuda. Antifungal effects of palmitic acid salt and ultrapure soft water on Scedosporium apiospermum. Journal of Appllied Microbiology 115(3): 711-7 (2013).
M. Sepahi, R. Jalal, and M. Mashreghi. Antibacterial activity of poly-l-arginine under different conditions. Iranian journal of microbiology 9(2): 103-111(2017).
K.G. Prasath, H. Tharani, M.S. Kumar, and S.K. Pandian. Palmitic Acid Inhibits the Virulence Factors of Candida tropicalis: Biofilms, Cell Surface Hydrophobicity, Ergosterol Biosynthesis, and Enzymatic Activity. Frontiers in Microbiology 11: 864(2020).
M. Taniguchi, A. Ochiai, K. Takahashi, S. Nakamichi, T. Nomoto, E. Saitoh, T. Kato, and T. Tanaka. Effect of alanine, leucine, and arginine substitution on antimicrobial activity against Candida albicans and action mechanism of a cationic octadecapeptide derived from α-amylase of rice, Peptide Science 106( 2): 219-229(2016).
C.B. Huang, Y. Alimova, T.M. Myers, and J. L. Ebersole. Short- and medium-chain fatty acids exhibit antimicrobial activity for oral
microorganisms. Archives of Oral Biology 56(7) : 650-654. (2011).
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