Screening of Natural Dyes from Selected Fungal Species

Analysis of Natural Dyes

Authors

  • Tasbiah Naz Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Shomaila Sikandar Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Fatima Sajjad Department of Life Sciences, University of Central Punjab, Lahore, Pakistan
  • Alim-un-Nisa Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore, Pakistan
  • Imran Afzal Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Anum Fatima Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Nayyar Rubab Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Rabia Sattar Department of Biology, Lahore Garrison University, Lahore, Pakistan

DOI:

https://doi.org/10.53560/PPASB(58-4)684

Keywords:

Cotton fabric, ecofriendly, natural pigments, characterization, antibacterial activity, dyeing

Abstract

Some fungi are observed as effective pigments. Its importance in the production of natural pigments has grown significantly. The pigment-producing fungi were developed and evaluated for application in dyeing cotton fabric. In the research, five fungal strains were identified as Aspergillus terreus S10, Talaromyces atroroseus WW5A3, Penicillium oxalicum WW3A4 (DG), WW5C2 and WW31DG. These strains were incubated for 21 days under static and non-static conditions using MSM and PDB media. Under liquid state fermentation conditions, the production of the pigments by the fungus was improved by altering temperatures (25-35°C) and pH (4.5-6.5). T. atroroseus WW5A3 showed pinkish color, A. terrreus S10 displayed yellow color, P. oxalicum WW3A4 (DG) presented yellowgreenish, WW5C2 exhibited light yellow color and WW31DG demonstrated greenish color. The results showed the maximum percentage absorbance of T. atroroteus WW5A3 showed 90.36 % at 600 nm, A. terrreus S10 showed 88 % at 500 nm, P. oxalicum WW35A4 showed 46.04 % at 550 nm, WW5C2 showed 59.60 % at 550 nm, and WW31DG showed 81.9 % at 550 nm. The natural fungal pigments were tested against bacterial pathogens to check the antibacterial activity. The results indicated that S. aureus and E. coli exhibited antibacterial activity in terms of maximum zone of inhibition. In conclusion, out of five pigments producing fungi, Aspergillus terreus S10 and Talaromyces atroroseus produced maximum pigment and highest percentage absorbance under liquid state fermentation conditions. Potential applications in the textile and leather industries have been discovered as a result of this research. 

References

T.M.A. Ghany. Safe Food Additives: A review. Journal of Biological and Chemical Research 32(1): 402-437 (2015).

M. Afshari, F. Shahidi, S.A. Mortazavi, F. Tabatabai and Z. Eshaghi. Investigating the influence of pH, temperature and agitation speed on yellow pigment production by Penicillium aculeatum ATCC 10409. Natural Product Research 29(14): 1300-1306 (2015).

S. Ali, T. Hussain, R. Nawaz. Optimization of alkaline extraction of natural dye from Henna leaves and it’s dyeing on cotton by exhaust method. Journal of Cleaner Production 17: 61-66 (2009).

J. Avalos and M.C. Limón. Biological roles of fungal carotenoids. Current Genetics 61(3): 309- 324 (2015).

P. Nigam, A. Pandey, S. Babitha. Microbial pigments. In: Biotechnology for agroindustrial residues utilization. Springer, Netherlands, p. 147- 162 (2009).

S. Babitha, C.R. Soccol and A. Pandey. Jackfruit seed - a novel substrate for the production of Monascus pigments throught solid-state fermentation. Food Technology and Biotechnology 44(4): 465-471(2007).

R.M. Buhler, B.L. Muler, D.E. Moritz, F. Vendruscolo, D. Oliveira and J.L. Ninow. Influence of light intensity on growth and pigment production by Monascus ruber in submerged fermentation. Applied Biochemistry and Biotechnology 176(5): 1277-1289 (2015).

S.N. Chattopadhyay, N.C. Pan, A.K. Roy, S. Saxena and A. Khan. Development of natural dyed jute fabric with improved colour yield and UV protection characteristics. Journal of the Textile Institute 104: 808-818 (2013).

W. Chen, Y. He, Y. Zhou, Y. Shao, Y. Feng, M. Li and F. Chen. Edible filamentous fungi from the species Monascus: early traditional fermentations, modern molecular biology, and future genomics. Comprehensive Review on Food Science Food Safety 14: 555-567 (2015).

N.O. Christiana. Production of food colourants by filamentous fungi. African Journal of Microbiology Research 10(26): 960–971 (2016).

Z. Chadni, M.H. Rahaman, I. Jerin, K.M.F. Hoque and M.A. Reza. Extraction and optimisation of red pigment production as secondary metabolites from Talaromyces verruculosus and its potential use in textile industries. Mycology 8(1): 48–57 (2017).

N.O. Christiana. Production of food colourants by filamentous fungi. African Journal of Microbiology Research 10(26): 960–971 (2016).

V.A. Hernán6dez, F. Galleguillos, R. Thibaut, and A. Müller. Fungal dyes for textile applications: testing of industrial conditions for wool fabrics dyeing. Journal of the Textile Institute 110(1): 61-66 (2019).

I. Holme. Sir William Henry Perkin: a review of his life, work and legacy. Color Technology 122: 235– 251 (2006).

S.J. Kadolph. Natural Dyes: A Traditional Craft Experiencing New Attention. Delta Kappa Gamma bull 75(1): 14-17 (2008).

K.D. Hyde, J. Xu, S. Rapior, R. Jeewon, S. Lumyong, A.G.T. Niego, P.D. Abeywickrama, J.V.S. Aluthmuhandiram, R.S. Brahamanage, S. Brooks,A. Chaiyasen, K.W.T. Chethana, P. Chomnunti, C. Chepkirui, B. Chuankid, N.I.D. Silva, M. Doilom, Faulds, E. Gentekaki and M. Stadler. The amazing potential of fungi: 50 ways we can exploit fungi industrially. Fungal Diversity 97(1): 1–136 (2019).

R. Mongkholrattanasit, J. Krystufek and J. Wiener. Dyeing and Fastness Properties of Natural Dyes Extracted from Eucalyptus Leaves Using Padding Techniques. Fiber and Polymers 11: 346-350 (2010).

S. Muthusamy, S. Udhayabaskar, G.P. Udayakumar, G.B. Kirthikaa and N. Sivarajasekar. Properties and Applications of Natural Pigments Produced from Different Biological Sources-A Concise Review. In: Sustainable Development in Energy and Environment. Springer, Singapore, p. 105-119 (2020).

F. Nourmohammadian and M.D. Gholami. An Investigation of the Dyeability of Acrylic Fiber Via Microwave Irradiation. Progress in Color, Colorants and Coatings Journal 1: 57-63 (2008).

S. Ratnapandian, S.M. Fergusson and L. Wang. Application of acacia natural dyes on cotton by pad dyeing. Fibers and Polymers 13: 206-211(2012).

A.C. Lagashetti, L. Dufossé, S.K. Singh, and P.N. Singh. Fungal pigments and their prospects in different industries. Microorganisms 7(12): 1-36 (2019).

H.S. Tuli, P. Chaudhary, V. Beniwal and A.K. Sharma. Microbial pigments as natural color sources: current trends and future perspectives. Journal of Food Science Technology 52(8): 4669- 4678 (2015).

F. Vendruscolo, R.M.M. Bühler, J.C. Carhalho, D.Oliverira, D.E. Moritz, W. Schmidell, J.L. Ninow. Monascus: A reality on the production and application of microbial pigments. Applied Biochemistry and Biotechnology 178(2): 211-223 (2015).

S. Venugopal, and Y. Khambhaty. Agro-residues for enhanced production of bio-colorants: a feasible approach for dyeing and surface coating of leather. Clean Technologies and Environmental Policy 22: 1687-1700 (2020).

S. Venugopal, Y. Khambhaty, K.Y. Choi, C.K. Venil, P. Velmurugan, L. Dufossé, P.R. Devi, A.V. Ravi, J.Polak, K. Wlizło, R. Pogni, E. Petricci, M. Grąz, K. Szałapata, M.O. Jaroszuk, J. Kapral‐piotrowska, B.P. Pawlęga, A.J. Wilkołazka, M.A. Kähkönen and P.E. Koehler. Exploring the potential of Epicoccum nigrum for pigment production under variable conditions. Indian Journal of Fibre and Textile Research 8(1): 555-562 (2020).

C.K. Venil and P. Lakshmanaperumalsamy. An insightful overview on microbial pigment, Prodigiosin. Electronic Journal of Biology 5(3): 49- 61 (2009).

R.E. Wrolstad. Anthocyanin Pigments Bioactivity and Coloring Properties. Journal of Food Sciences 69: 419-425 (2004).

Y.J. Cho, J.P. Park, H.J. Hwang, S.W. Kim, J.W. Choi and J.W. Yun. Production of red pigment by submerged culture of Paecilomyces sinclairii. Letters in Applied Microbiolology 35: 195-202 (2002).

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Published

2021-12-18

How to Cite

Naz, T. ., Sikandar, S. ., Sajjad, F. ., Alim-un-Nisa, Afzal, I. ., Fatima, A. ., Rubab, N. ., & Sattar, R. . (2021). Screening of Natural Dyes from Selected Fungal Species: Analysis of Natural Dyes. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 58(4), 39–48. https://doi.org/10.53560/PPASB(58-4)684

Issue

Vol. 58 No. 4 (2021)

Section

Research Articles

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