In vitro Propagation of Potato Cultivar under Abiotic Stress

Potato propagation under abiotic stress

Authors

  • Arifa Khan International Islamic University, Islamabad, Pakistan
  • Naveeda Riaz International Islamic University, Islamabad, Pakistan
  • Shazia Erum Bio Resources Conservation Institute, NARC, Islamabad, Pakistan
  • Muhammad Hanif Potato Program, NARC, Islamabad, Pakistan
  • Waqas Ahmed Potato Program, NARC, Islamabad, Pakistan
  • Nadeem Iqbal University of Ponch, Rawalakot, AJ&K, Pakistan
  • Abdullah International Islamic University, Islamabad, Pakistan
  • Shabana Irum Bio Resources Conservation Institute, NARC, Islamabad, Pakistan
  • Sajida Naeem International Islamic University, Islamabad, Pakistan

Keywords:

Potato, propagation, Environmental factors, Explant

Abstract

The research study was conducted to explore the abiotic effects on in vitro propagation of “Karuda” potato cultivar. Potato shoot tips were cultured on Murashige and Skoog medium (MS media) with varying pH (4.8-8.8). Best results were shown when explant was cultured on MS medium with adjusted pH 5.8 with numbers of nodes (8), shoot length (6.5 cm), numbers of leaves (7), numbers of roots (2), followed by numbers of nodes (3), shoot length (4.5 cm), numbers of leaves (2), numbers of roots (1) at pH 5.8. Different light conditions and temperature variations were also examined. The results declared that there was a significant difference in numbers of nodes (4), shoot length (4.9 cm), numbers of leaves (4.7), numbers of roots (1) were more at 25°C followed by numbers of nodes (3), shoot length (1.5), numbers of leaves (7), numbers of roots (3) at 30 °C while negligible growth was recorded at 40°C. The 2 mg/L BAP cytokinin+0.1 mg/L NAA showed maximum growth in potato. Moreover, this study also showed that light and dark conditions also affected plant growth. It is evident that environmental factors pose a great impact on plant growth.

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Published

2020-06-11

How to Cite

Khan, A. ., Riaz, N., Erum, S. ., Hanif, M., Ahmed, W. ., Iqbal, N. ., Abdullah, Irum, S. ., & Naeem, S. (2020). In vitro Propagation of Potato Cultivar under Abiotic Stress: Potato propagation under abiotic stress. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 57(2), 75–82. Retrieved from https://ppaspk.org/index.php/PPAS-B/article/view/41

Issue

Vol. 57 No. 2 (2020)

Section

Research Articles

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