Spectrophotometric Evaluation of the Sun Protection Factor of Wild Sideritis raeseri Extracts under Different Solvent and Temperature Conditions
DOI:
https://doi.org/10.53560/PPASB(63-1)1127Keywords:
Sideritis raeseri, SPF, UV Protection, Natural Sunscreen, Mansur MethodAbstract
Plant-origin sunscreen products for skin protection from ultraviolet radiation are preferred over synthetic products as safer with regard to possible toxicity, allergies, and are eco-friendly. This study fills a gap in peer review research for endemic Albanian wild mountain tea (Sideritis raeseri Boiss & Heldr.) for its ultraviolet photoprotective properties under various solvent types, incubation days, and temperatures, by utilizing the Mansur method. S. raeseri aqueous extracts were centrifuged, filtered, and then mixed in two Erlenmeyer flasks: one with distilled water and the other with ethanol in a 1:4 v/v ratio. Absorbance measurements within the 290-320 nm range were taken at different temperatures over six consecutive days. Neither water nor ethanol absorbs within this range. In extracts at room temperature (29-31 °C), Sun Protection Factor (SPF) value expressed as mean ± standard deviation dropped from 26.426 ± 0.2 on day one to 17.930 ± 0.19 on day six; for refrigerated extracts at 11 °C, SPF dropped from 26.426 ± 0.2 to 22.469 ± 0.19. In the water-ethanolic mixture at room temperature, SPF dropped from 23.281 ± 0.21 to 13.387 ± 0.036, and with refrigeration, from 23.281 ± 0.21 to 21.199 ± 0.21. At refrigerated conditions, the SPF of S. raeseri extract was photochemically more stable and showed a longer shelf-life, while SPF values at room temperature incubation were lower. Higher SPF values were found in water solutions than water-ethanol. The refrigerated water-ethanol mixture could be utilized in sunscreen products as a stability factor for SPF values of S. raeseri extracts.
References
1. M.R. Fabris, E.S.M. Durães, B.C.D.F. Martignago, L.F.D.O. Blanco, and T.R. Fabris. Assessment of knowledge of skin cancer prevention and its relationship with sun exposure and photo protection among gym academy members in the south of Santa Catarina, Brazil. Anais Brasileiros Dermatology 87: 36-43 (2012). https://doi.org/10.1590/S0365-05962012000100004
2. R.R. Korać and K.M. Khambholja. Potential of herbs in skin protection from ultraviolet radiation. Pharmacognosy Reviews 5: 164-173 (2011). https://doi.org/10.4103/0973-7847.91114
3. E.W. Breitbart, R. Greinert, and B. Volkmer. Effectiveness of information campaigns. Progress in Biophysics and Molecular Biology 92: 167-172 (2006). https://doi.org/10.1016/j.pbiomolbio.2006.02.023
4. J.R. Raymond-Lezman and S.I. Riskin. Sunscreen Safety and Efficacy for the Prevention of Cutaneous Neoplasm. Cureus 16(3): e-56369 (2024) https://doi.org/10.7759/cureus.56369
5. V.K. Pal, S. Lee, and K. Kannan. Occurrence of and dermal exposure to benzene, toluene and styrene in sunscreen products marketed in the United States. Science of The Total Environment 888: 164196 (2023) https://doi.org/10.1016/j.scitotenv.2023.164196
6. S. Suh, C. Pham, J.Smith, and N.A.Mesinkovska. The banned sunscreen ingredients and their impact on human health: a systematic review. International Journal of Dermatol, 59: 1033-1042 (2020) https://doi.org/10.1111/ijd.14824
7. N.J. Wheate. A review of environmental contamination and potential health impacts on aquatic life from the active chemicals in sunscreen formulations. Australian Journal of Chemistry 75: 241–248 (2022) https://doi.org/10.1071/CH21236
8. E.A. Dutra, D.A.G. da Costa e Oliveira, E.R.M. Kedor-Hackmann, and M.I.R.M. Santoro. Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Brazilian Journal of Pharmaceutical Sciences 40(3): 381 (2004) https://doi.org/10.1590/S1516-93322004000300014
9. M. Zarkogianni and N. Nikolaidis. Determination of Sun Protection Factor (SPF) and Stability of Oil-in-Water Emulsions Containing Greek Red Saffron (Crocus Sativus L.) as a Main Antisolar Agent. International Journal of Advanced Research in Chemical Science 3(7): 1-7 (2016). http://dx.doi.org/10.20431/2349-0403.0307001
10. C. Stiefel and W. Schwack. Photoprotection in changing times - UV filter efficacy and safety, sensitization processes and regulatory aspects. International Journal of Cosmetic Science 37(1): 2-30 (2015). https://doi.org/10.1111/ics.12165
11. M. Abou-Dahech, A. Schaefer, L. Lam-Phaure, A.N. Huynh, M. Chandler, and G. Baki. Effect of Solvents on the In Vitro Sun Protection Factor and Broad-Spectrum Protection of Three Organic UV Filters. Journal of Cosmetic Science 71(3):149-165 (2020). https://pubmed.ncbi.nlm.nih.gov/33022210/
12. J.D. Mason, M.T. Cone, and E.S. Fry. Ultraviolet (250–550 nm) absorption Spectrum of pure water. Applied Optics 55: 7163-7172 (2016). https://doi.org/10.1364/AO.55.007163
13. V. Shalatonin. Water-SARS-CoV-2 Interaction-Based Mechanism Inhibiting Virus Attachment to Host Cells. ChemRxiv, Biological and Medicinal Chemistry. A preprint that has not been peer reviewed (2020). https://doi.org/10.26434/chemrxiv.13443392.v1
14. H. Saad, M.A Rahman, I. Yassin, and A.M. Muad. Characterization of ethanol concentrations at ultraviolet wavelength region. Journal of Fundamental and Applied Sciences 9(4S): 384-400 (2018). https://www.ajol.info/index.php/jfas/article/view/165313
15. E. Hodaj-Çeliku, O.Tsiftsogloua, L. Shuka, S. Abazi, D. Hadjipavlou-Litinaf, and D. Lazari. Antioxidant Activity and Chemical Composition of Essential Oils of some Aromatic and Medicinal Plants from Albania. Natural Product Communication 12(5): 785-790 (2017). https://doi.org/10.1177/1934578X1701200525
16. John Wiley & Sons, Inc. Spectrabase https://spectrabase.com/spectrum/32Hmd4OFCpI
17. John Wiley & Sons, Inc. Spectrabase https://spectrabase.com/spectrum/1zFrwZ5GI2Z
18. D. Żyżelewicz, K. Kulbat-Warycha, J. Oracz, and K. Żyżelewicz. Polyphenols and Other Bioactive Compounds of Sideritis Plants and Their Potential Biological Activity. Molecules 25(16): 3763 (2020). https://doi.org/10.3390/molecules25163763
19. V. Romanucci, G. Di Fabio, D. D'Alonzo, A. Guaragna, G. Scapagnini, and A. Zarrelli. Traditional uses, chemical composition and biological activities of Sideritis raeseri Boiss. & Heldr. Journal of Science of Food and Agriculture 97: 373-383 (2017). https://doi.org/10.1002/jsfa.7867
20. J.C Espín, C. Soler-Rivas, and H.J.Wichers. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. Journal of Agricultural and Food Chemistry 48: 648-56 (2000). https://doi.org/10.1021/jf9908188
21. C. Couteau, A. Philippe, A. Ali, M. Bernet, M. Lecoq, E. Paparis, and L. Coiffard. Study of the influence of alcohol on the photostability of four UV filters. European Review for Medical and Pharmacological Science 25: 6025-6033 (2021). https://doi.org/10.26355/eurrev_202110_26880
22. European Union Regulation (EC) N° 1223/2009 of the European Parliament and the Council on cosmetic products. Document 32009R1223. Official website of the European Union (2009). http://data.europa.eu/eli/reg/2009/1223/oj
23. R.U. Pendlington, E. Whittle, J.A. Robinson, and D. Howes. Fate of ethanol topically applied to skin. Food and Chemical Toxicology 39: 169-174 (2001). https://doi.org/10.1016/S0278-6915(00)00120-4
24. A. Kramer, H. Below, N. Bieber, G. Kampf, C.D. Toma, N.O. Huebner, and O. Assadian. Quantity of ethanol absorption after excessive hand disinfection using three commercially available hand rubs is minimal and below toxic levels for humans. BMC Infectious Diseases 7: 117 (2007). https://doi.org/10.1186/1471-2334-7-117
25. G.L. Salazar-Orbea, R. García-Villalba, M.J. Bernal, A. Hernández-Jiménez, J.A. Egea, A.F. Tomás-Barberán, and L.M. Sánchez-Siles. Effect of Storage Conditions on the Stability of Polyphenols of Apple and Strawberry Purees Produced at Industrial Scale by Different Processing Techniques. Journal of Agricultural and Food Chemistry 71(5): 2541-2553 (2023). https://pubs.acs.org/doi/10.1021/acs.jafc.2c07828
26. A.N. Pavlović, J.M. Mrmošanin, J.N. Krstić, S.S. Mitić, S.B. Tošić, M.N. Mitić, B.B. Arsic, and R.J. Micic. Effect of storage temperature on the decay of catechins and procyanidins in dark chocolate. Czech Journal of Food Sciences 35(4): 360-366 (2017). https://cjfs.agriculturejournals.cz/pdfs/cjf/2017/04/09.pdf
27. R.M. Soliman, R.A.A. Salam, B.G. Eid, A. Khayyat, T.N. Neamatallah, M.K. Mesbah, and G. Hadad. Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS. Acta Pharmaceutica. 70: 325-342 (2020). https://doi.org/10.2478/acph-2020-0028
28. A. Plaskova and J. Mlcek. New insights of the application of water or ethanol-water plant extract rich in active compounds in food. Frontiers in Nutrition 10: 1118761 (2023). https://doi.org/10.3389/fnut.2023.1118761
29. M. Shirali, S. Rouhani, K. Gharanjig, and F. Mirhashemi. Exploring solvatochromism: a comprehensive analysis of research data of the solvent -solute interactions of 4-nitro-2-cyano-azo benzene-meta toluidine. BMC Chemistry 18: 154 (2024). https://doi.org/10.1186/s13065-024-01249-5
30. M.V. Galmarini, M. Chantal, E. Mehinagic, V. Sanchez, R. Baeza, S. Mignot, M. Zamora, and J. Chirife. Stability of Individual Phenolic Compounds and Antioxidant Activity During Storage of a Red Wine Powder. Food and Bioprocess Technology 6: 3585-3595 (2013). https://doi.org/10.1007/s11947-012-1035-y
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