Isolation of Monochloroacetic Acid Biodegrading Bacteria from Tigris River
Monochloroacetic acid biodegrading bacteria fromTigris River
DOI:
https://doi.org/10.53560/PPASB(59-4)753Keywords:
Pseudomonas sp SW2, Dehalogenase, Monochloroacetic (MCA), Degradation xenobioticAbstract
Organic compounds containing halogens are widely dispersed throughout the world, resulting in different types of pollution. One of the most common xenobiotics used in agricultural activities is monochloroacetic (MCA) which was Isolated from Iraqi mud in the Tigris River. This bacterial strain was known as SW2. Both standard universal primers Fd1 and rP1 were used with the colony PCR method for bacterial identification before being sent out for sequencing. Basic Local Alignment Search Tool nucleotide sequences and information were analyzed (BLASTn). The phylogenetic tree was constructed using the 16S rRNA sequence to determine their evolutionary distance. The Neighbor-Joining method was used to infer the evolutionary history. The Neighbor-Joining method was used to infer the evolutionary history. There is a 96 percent match between the SW2 bacterium and another type of aerobic GramNegative Bacteria. Strain SW2 (Pseudomonas sp.) was inoculated for two days and yielded colonies that were small, non-spore-forming, and rod-shaped. Growth slowed slightly after 48 hours. The release of chloride ions as a result of the degradation of MCA was seen using a halide ion test. Biochemical tests backed up the choice of the genus’s name as well. As a result, bacteria found in the river are capable of utilizing and degrading the MCA compound. In conclusion, this study confirmed the potential ability of the isolated bacterial strain of utilizing MCA, especially from contaminated environments with the pragmatic application of the bacterial strains to degrade residual herbicide.
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