Impact of Stevia rebaudiana Culturing in Liquid Medium: Elevation of Yield and Biomass, Mitigation of Steviol Glycosides
Comparative analysis of culturing of Stevia rebaudiana in solid and liquid media
DOI:
https://doi.org/10.53560/PPASB(59-1)704Keywords:
Stevia rebaudiana, Micropropagation, Liquid Culturing, Steviol GlycosidesAbstract
An efficient micropropagation system is developed by culturing nodal segments containing the axillary shoot buds on liquid Murashige and Skoog (MS) medium. Direct shoot and root formation are highly effective in both solid and liquid MS media without any plant growth regulators (PGRs). Interestingly, a significant difference in yield is obtained between solid and liquid cultures. It is revealed that a relatively higher amount of plant biomass is obtained after culturing for 4 weeks in a liquid MS medium. However, the shoots produced on solid MS medium produce a remarkable decline in all physiological parameters. On contrary, the bioactive steviol glycosides (rebaudioside-A and stevioside) content is higher in shoots grown in solid MS on a comparative basis, which could be compensated by higher yield. After hardening off, all the regenerants are effectively grown in the field with a negligible loss (<1 %), and steviol glycosides spectra is again obtained by conducting high-performance liquid chromatography (HPLC) analysis after 10 weeks of the plantation. This method has great potential to be applied on large scale in bioreactors.
References
A.D. Kinghorn. Stevia: the genus Stevia. Taylor and Francis Inc., NY, p. 1–17 (2002).
D. Soejarto. Botany of Stevia and Stevia rebaudiana. Taylor and Francis, In: A. Kinghorn (Ed.), Stevia: The genus Stevia. London, New York, p. 18–39 (2002).
J.C. Liu, P.K. Kao, P. Chan, Y.H. Hsu, C.C. Hou, G.S. Lien, M.H. Hsieh, Y.J. Chen, and Y.T. Cheng. Mechanism of the antihypertensive effect of stevioside in anesthetized dogs. Pharmacology 67: 14 (2003).
A. Dey, S. Kundu, A. Bandyopadhyay, and A.Bhattacharjee. Efficient micropropagation and chlorocholine chloride induced stevioside production of Stevia rebaudiana Bertoni. CR Biologies 336: 17–28 (2013).
A.D. Anton, C.K. Martin, H. Han, S. Coulon, W.T. Cefalu, P. Geiselman, and Williamson DA. Effects of Stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. Appetite 55: 37–43 (2010).
U. Wölwer-Rieck. The leaves of Stevia rebaudiana (Bertoni), their constituents and the analyses thereof: a review. Journal of Agricultural and Food Chemistry 60: 886–895 (2012).
M. Libik-Konieczny, E. Capecka, M. Tuleja, and R. Konieczny. Synthesis and production of steviol glycosides: recent research trends and perspectives. Applied Microbiology and Biotechnology 105: 3883–3900 (2021).
A.K. Yadav, S. Singh, D. Dhyani, and P.S. Ahuja. A review on the improvement of Stevia [Stevia rebaudiana (Bertoni)]. Canadian Journal of Plant Science 91: 1–27 (2011).
Y. Tamura, S. Nakamura, H. Fukui, and M. Tabata. Clonal propagation of Stevia rebaudiana Bertoni by stem-tip culture. Plant Cell Reports 3: 183–185 (1984).
R. Javed, M. Usman, B. Yucesan., M. Zia, E. Gurel. Effect of zinc oxide (ZnO) nanoparticles on physiology and steviol glycosides production in micropropagated shoots of Stevia rebaudiana Bertoni. Plant Physiology and Biochemistry 110: 94–99 (2017a).
R. Javed, A. Mohamed, B. Yucesan, G. Ekrem, R. Kausar, and M. Zia. CuO nanoparticles significantly influence in vitro culture, steviol glycosides, and antioxidant activities of Stevia rebaudiana Bertoni. Plant Cell Tissue Organ Culture 131: 611–620 (2017b).
R. Javed, B. Yucesan, M. Zia, and E. Gurel. Differential effects of plant growth regulators on physiology, steviol glycosides content, and antioxidant capacity in micropropagated tissues of Stevia rebaudiana. Biologia 72: 1156–1165 (2017c).
R. Javed, B. Yucesan, and E. Gurel. Hydrogen peroxide-induced steviol glycosides accumulation and enhancement of antioxidant activities in leaf tissues of Stevia rebaudiana Bertoni. Sugar Tech 20: 100–104 (2018a).
R. Javed, B. Yucesan., M. Zia, and E. Gurel. Elicitation of secondary metabolites in callus cultures of Stevia rebaudiana Bertoni grown under ZnO and CuO nanoparticles stress. Sugar Tech 20: 194–201 (2018b).
R. Javed, and E. Gurel. Salt stress by NaCl alters the physiology and biochemistry of tissue culture- grown Stevia rebaudiana Bertoni. Turkish Journal of Agriculture and Forestry 43: 11–20 (2019).
M.A. Ahmad, R. Javed, M. Adeel, M. Rizwan, Q. Ao, and Y. Yang. Engineered ZnO and CuO nanoparticles ameliorate morphological and biochemical response in tissue culture regenerants of Candyleaf (Stevia rebaudiana). Molecules 25: 1356 (2020a).
M.A. Ahmad, R. Javed, M. Adeel, M. Rizwan, and Y. Yang. PEG 6000-stimulated drought stress improves the attributes of in vitro growth, steviol glycosides production, and antioxidant activities in Stevia rebaudiana Bertoni. Plants 9: 1552 (2020b).
M.A. Ahmad, X. Deng, M. Adeel, M. Rizwan, N. Shakoor, Y. Yang, and R. Javed. Influence of calcium and magnesium elimination on plant biomass and secondary metabolites of Stevia rebaudiana Bertoni. Biotechnology and Applied Biochemistry (2021).
H. Tavakoli, N. Tavakoli, and F. Moradi. The effect of the elicitors on the steviol glycosides biosynthesis pathway in Stevia rebaudiana. Functional Plant Biology 46: 787–795 (2019).
T. Murashige, and F.A. Skoog. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology 15: 473–497 (1962).
R.V. Sreedhar, L. Venkatachalam, R. Thimmaraju, N. Bhagyalakshmi, M.S Narayan, and G.A. Ravishankar. Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. Biologia Plantarum 52: 355–360 (2008).
K. Jiménez-Quesada. Evaluación del desarrollo in vitro de Stevia rebaudiana Bertoni con el empleo de sistemas de micropropagación semi-sólido, inmersión temporal RITA® y biorreactor de burbujeo. BSc in biology Thesis School of Biology, Costa Rica Institute of Technology, Costa Rica (in Spanish) (2011).
J. Striedner, F. Czygan, and Braunegg G. Contributions to the biotechnological production of sweeteners from Stevia rebaudiana Bertoni: a method for the serial analysis of diterpene glycosides by HPLC. Acta Biotechnologica 11: 495–499 (1991).
N. Bondarev, O. Reshetnyak, and A. Nosov. Effects of nutrient medium composition on development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides. Plant Science 165: 845–850 (2003).
B. Yücesan, A. Mohammed, R. Büyükgöçmen, A. Cevher, O. Kavas, S. Gürel, and E. Gürel. In- vitro and ex-vitro propagation of Stevia rebaudiana Bertoni with high Rebaudioside-A content—A commercial scale application. Scientia Horticulturae 203: 20–28 (2016).
S.M. Swanson, G.B. Mahady, and C.W.W. Beecher. Stevioside biosynthesis by callus, root, shoot and rooted-shoot cultures. Plant Cell Tissue Organ Culture 28: 151–157 (1992).
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