Effect of Dilute Sulphuric Acid Pretreatment on Cellulase Production by Bacillus subtilis (K-18) through Response Surface Methodology

Cellulase Production by Bacillus subtilis in Submerged Fermentation

Authors

  • Anam Arooj Microbial Biotechnology Laboratory, Department of the Zoology, University of the Punjab, Lahore, Pakistan
  • Muhammad Irfan Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
  • Fouzia Tabbasum Microbial Biotechnology Laboratory, Department of the Zoology, University of the Punjab, Lahore, Pakistan
  • Hafiz Abdullah Shakir Microbial Biotechnology Laboratory, Department of the Zoology, University of the Punjab, Lahore, Pakistan
  • Javed Iqbal Qazi Microbial Biotechnology Laboratory, Department of the Zoology, University of the Punjab, Lahore, Pakistan

Keywords:

Cellulase, RSM, pretreatment, Bacillus subtilis, submerged fermentation

Abstract

The present study investigated the optimization of dilute sulphuric acid pretreatment to maximize cellulase production from banana peduncle waste through Box-Behnken design of response surface methodology. Cellulase production was carried out in 250ml capacity Erlenmeyer flask using pretreated banana peduncle as substrate in submerged fermentation by Bacillus subtilis K-18 incubateda t 50o C for fermentation period of 24 h. Results indicated that chemical pretreatment using sulphuric acid favored cellulase production as compared to thermochemical pretreatment using sulphuric acid followed by autoclaving at 121o C for 15 min and 15 psi. Maximum Filter Paper activity of 0.958 IU/ml/min was observed at optimal pretreatment conditions of 0.4 N H2SO4 concentration, 15% substrate concentration and residence time of 6h with chemical pretreatment. For thermochemical
pretreatment optimal FPase activity of 0.63 IU/ml/min was recorded at 0.4 N H2SO4 concentration, 10% substrate concentration and residence time of 4 h. The proposed regression model for both types of pretreatments was found significant as revealed by F-value, P-value and coefficient of determination. These results indicated that banana peduncle can be successfully utilized as solid
substrate in submerged fermentation for cellulase enzyme production.

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Published

2017-03-18

How to Cite

Arooj, A. ., Irfan, . M., Tabbasum, . F., Shakir, H. A. ., & Qazi, . J. I. . (2017). Effect of Dilute Sulphuric Acid Pretreatment on Cellulase Production by Bacillus subtilis (K-18) through Response Surface Methodology: Cellulase Production by Bacillus subtilis in Submerged Fermentation. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 54(1), 11–. Retrieved from http://ppaspk.org/index.php/PPAS-B/article/view/373

Issue

Vol. 54 No. 1 (2017)

Section

Research Articles

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