Alpha Mangosteen Effect on MDA Level and the Pancreatic Morphology Rattus norvegicus (Berkenhout, 1769) Induced by Alloxan
Alpha Mangosteen Effect on MDA Level and the Pancreatic Morphology
Keywords:
Beta cells, Diabetes mellitus, Garcinia mangostana L., Lipid peroxidationAbstract
The alpha mangosteen compound is a single compound isolated from rind extract Garcinia mangostana Linn, known to have antidiabetic bioactivity. This research aimed to determine the effect of the α-mangosteen compound on Malondialdehyde (MDA) and rats pancreatic tissue induced with type 2 Diabetes mellitus. Twentyfour Wistar male rats were divided into four groups, each group consisting of six rats. The first group was set as a positive control, rats injected with a single dose of 150 mg kg–1 alloxan. The second group was the negative control, which means that no treatment. The third group was injected with a single dose of 150 mg kg–1 alloxan and α-mangosteen compound 10 mg kg–1, while the last group was injected with a single dose of 150 mg kg–1 alloxan and 10 mg kg–1 standard drug glibenclamide. All groups were monitored for 3 wk, and blood sugar levels were measured using a Glucose meter. Blood samples were taken for the measurement of MDA, and the pancreas organ was removed for histological study. This research showed that the difference of MDA level between α-mangosteen compound
treatment and glibenclamide was only 0.481 8. It means that the α-mangosteen compound can reduce MDA levels alike the standard drug glibenclamide capabilities. Islets of Langerhans on normal pancreatic mice (control (-)) looked filling full with the endocrine cells spread throughout the pancreas. While at the control group (+) there was a roomempty space (fewer the number of beta cells) in islets of Langerhans. On the treatment groups, those were giving α-mangosteen, and drug compounds standard (glibenclamide) showed improvement in Langerhans β cells compared with a treatment group of diabetic mice (control+). The administration of α-mangosteen compound 10 mg kg–1 BW able to decrease MDA level 1.204 7 nmol dl–1. While based on histological observations of the pancreas showed that the alpha mangosteen treatment group showed improvement of Langerhans β cells compared to the diabetic rat group (positive control)
References
R.I. Depkes. Pharmaceutical Care untuk Penyakit Diabetes Mellitus. [Pharmaceutical Care for Diabetes mellitus]. Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan, Jakarta (2013).
A. Husni., T. Pratiwi., Ustadi., A.G. Samudra, and A.E. Nugroho. In vitro antidiabetic activity of Sargassum hystrix and Eucheuma denticulatum from Yogyakarta Beach of Indonesia. Proceedings of the Pakistan Academy of Sciences: Pakistan Academy of Sciences B. Life and Environmental Sciences 55 (3): 1–8 (2018).
A.C. Melviana., R.R. Esyanti., R.H. Setyobudi., M. Mel., P.G. Adinurani, and J. Burlakovs. Gene expression related to steviol glycoside synthesis produced in Stevia rebaudiana (Bert.) shoot culture induced with high far-red LED light in TIS RITA® bioreactor system. Sarhad Journal of Agriculture 37(1): 1–8 (2021).
D.D. Wulandari, and T. Ersam. Study of α-Mangosteen compound and antidiabetic assay from fruit hull of Garcinia mangostana Linn.
International Seminar on Medicinal Chemistry and Timmerman Award. 1–5 (2011).
E.I. Mohamed., A. Elazomi., B.E.H. Elabid, and H. Zwaik. Evaluation of changes in levels of plasma MDA, and antioxidant vitamin E in Sudanese patients with type2 diabetes. International Conference on Chemical, Environment & Biological Sciences, September 17–18, 2014 Kuala Lumpur, Malaysia, p. 1–4 (2014).
M. Ajibola., M. Eunice, and I.M. Stephanie. Effects of aqueous extract of moringa oleifera seeds on alloxan induced hyperglycemia. asic Sciences of Medicine 3(3): 37–42 (2014).
A. Rai., C. Eapen, and V.G. Prasanth. Interaction of herbs and glibenclamide: A review. International Scholarly Research Network Pharmacology 2012: 1–5 (2012).
F.A. Dawud., E.D. Eze., A.A. Ardja., A.S. Isa., A. Jimoh., M. Bashiru, and I.S. Malgwi. Ameliorative effects of vitamin C and Zinc in alloxan-induced diabetes and oxidative stress in Wistar rats. Current Research Journal of Biological Sciences 4(2): 123–129 (2012).
A. Antonio., M.F. Muñoz, and S. Argüelles. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Medicine and Cellular Longevity 2014: 1–31 (2014).
B.K. Tiwari., K.B. Pandey., A.B. Abidi, and R.I. Syed. Markers of oxidative stress during Diabetes mellitus. Journal of Biomarkers 2012: 1–8 (2013).
M. Thakur, and J. Dinesh. Adenosine deaminase and Amalondialdehyde levels in Type-2 Diabetes mellitus: A short study. Global Journal of Medical Research 15(3): 1–5 (2014).
O.O. Erejuwa. Management of Diabetes mellitus: Could simultaneous targeting of hyperglycemia and oxidative stress be a better panacea? International Journal of Molecular Science 13: 2965–2972 (2012).
M. Buelna-Chontal., F. Correa., S. Herna´ndezRese´ndiz., Z. Zazueta, and J. Pedraza-Chaverri. Protective effect of α-Mangosteen on cardiac reperfusion damage by attenuation of oxidative stress. Journal of Medicinal Food 14(11): 1370–1374 (2011).
A.C. Carrano., F. Mulas., C. Zeng, and S. Maike. Interrogating islets in health and disease with singlecell technologies. Molecular Metabolism 6(9): 991–1001 (2017).
G.D.S. Xavier. The cells of the islets of langerhans. Journal of Clinical Medicine 7(3): 54 (2018).
D. Lin., M. Xiao., J. Zhao., Z. Li., B. Xing., X. Li., M. Kong., L. Li., Q. Zhang., Y. Liu., H. Chen., W. Qin., H. Wu, and S. Chen. An overview of
plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules 21(10): 1374 (2016).
M.N. Sarian., Q.U. Ahmed., S.Z.M. So’ad., A.M. Alhassa., S. Murugesu., V. Peruma., S.N.A.S Mohamad., A. Khatib, and L. Jalifah. Antioxidant and antidiabetic effects of flavonoids: A Structureactivity relationship-based study. BioMed Research International 2017: 1–14 (2017).
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