Enhanced Production of Biosurfactant by Pseudomonas aeruginosa Isolate GW-7 under Nitrogen and Phosphate Limiting Conditions
Biosurfactant Production by Pseudomonas aeruginosa Isolate GW-7
Keywords:
Bacteria, biosurfactant production, characterization, nitrogen and phosphate limitation, culture mediaAbstract
novel bacterial strain of Pseudomonas aeruginosa has been isolated and characterized by screening samples from formation water of local oil wells and other habitat, which overproduced potent biosurfactant under phosphate limiting condition within 36 hours of incubation. Biosurfactant producing bacteria were isolated from the oil, formation water and from the soil contaminated with oil from the local oil wells; strains were grown on different medias having different carbon sources. Selected 66% culture reduced surface tension lesser than 33 mN/m and interfacial tension lesser than 3 mN/m whereas 85% showed hemolytic activity. Pseudomonas spp. was most common while some Micrococcus and Acinetobacter were also identified. Most potent biosurfactant producers were P. aeruginosa, P. fluorescens and Micrococcus spp. P. aeruginosa strain GW-7 was selected for further studies. BHmineral medium was best for biosurfactant production as it reduced surface tension to (32 mN/m), interfacial tension
(2.5 mN/m).Other best medium was an inorganic phosphate limited medium containing proteose peptone/glucose/ammonium salts, as its surface tension and interfacial tension reached to 30 mN/m and 2.5 mN/ m, respectively, after 36 hours. P. aeruginosa isolate GW-7 isolated from oil enriched soils from Gwadar coastal area produced potent biosurfactant (rhamnolipid) in large amount in reasonably short period of time (36 hours) under nitrogen and phosphate limiting conditions.
References
Yilmaz, F. A., E. Ergene, S. Yalçin, & Tan. Production and characterization of biosurfactants produced by microorganisms isolated from milk factory wastewaters. Environmental Technology 30(13): 1397-1404 (2009).
Cameotra, S.S., R. S. Makkar., J. Kaur. & S.K. Mehta. Synthesis of biosurfactants and their advantages to microorganisms and mankind.
Advanced Experimental Medical Biology 672: 261- 280 (2010).
Banat, I.M., A. Franzetti., I. Gandolfi, G. Bestetti. & M. G. Martinotti. Microbial biosurfactants production, applications and future potential. Applied Microbial Biotechnology 87:427-444 (2010).
Banat, I.M., S.K. Satpute., S.S. Cameotra., R. Patil. & N.V. Nyayanit. Cost effective technologies and renewable substrates for biosurfactants production. Frontiers of Microbiology 5:697-707 (2014).
Nguyen, T.T. & D.A. Sabatini. Characterization and emulsification properties of rhamnolipid and Sophorolipid biosurfactants and their applications. International Journal of Molecular Science 12: 1232-1244 (2011).
Randhawa, K. & P. Rahman. Rhamnolipid biosurfactants: past, present & future scenario of global Market. Frontiers of Microbiology 5:454 (2014).
Banat, I.M. & S.S. Cameotra. Potential commercial applications of microbial surfactants. Applied Microbial Biotechnology 53:495–508 (2000).
Nguyen, T.T & N.H. Youssef. Rhamnolipid biosurfactant mixtures for environmental remediation. Water Research 42:1735–1743 (2008).
Eleftheria, A., F. Stilianos, K. Emmanouela, K. Nicolas. Biosurfactant production from marine hydrocarbon degrading consortia and pure bacterial strains using crude oil as carbon source. Frontiers of Microbiology 6:1-14 (2015).
Benincasa, M., A. Abalos, I. Moreira, A. Manresa. Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source. Journal of Food Engineering 54:283–288 (2002).
Haba, E., M.J. Espuny, M. Busquets, A. Manresa. Screening and production of Rhamnolipids by Pseudonomas aeruginosa 47T2 NCIB 40044 from waste frying oils. Journal of Applied Microbiology 88:379-387 (2000).
Mulligan, C.N., G. Mahmourdis, B.F. Gibbs. The influence of phosphate metabolism on biosurfactant production by Pseudomonas aeruginosa. Journal of Biotechnology 12 (3–4):199-209 (1989b).
Bazire, A., A. Dheilly, D. Haras, A. Dufour. Osmotic stress and phosphate limitation alter production of cell-to-cell signal molecules and rhamnolipid biosurfactant by Pseudomonas aeruginosa. FEMS Microbiology Letters 253(1):125-31 (2005).
Clarke, K.G., F. Ballot, S.J. Reid. Enhanced rhamnolipid production by Pseudomonas aeruginosa under phosphate limitation. World Journal of Microbiology and Biotechnology. 26(12):2179-2184 (2010).
Bushneel, L.D & H.E. Haas. Utilization of certain hydrocarbons by microorganisms. Journal of Bacteriology 41:653-673 (1941).
Finnerty, W.R & M.E. Singer. A microbial biosurfactant Physiology, Biochemistry &Applications. Indian Journal of Microbiology
:31-40 (1984).
Mulligan, C.N., D.G. Cooper, R. J. Neufeld. Selection of microbes producing biosurfactant in media without Hydrocarbons. Journal of
Fermentation Technology 62(4):311-314 (1984).
Khalid, Z.M & K.A. Malik. Isolation of Furnace Oil utilizing bacteria capable of producing biosurfactant. Pakistan Journal Scientific & Industrial Research 31(10):714-717 (1988).
Guerra–Santos, L., O. Kappeli, A. Fiechter. Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source. Applied Environmental Microbiology 48:301-305(1984).
Kavocs, N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature. 178:703-708 (1956).
Enfors, S.O., G. Molin, A. Ternstrom. Effect of packaging under CO2, nitrogen or air on the microbial flora of pork stored at 400 C. Journal of Applied Bacteriology 47:197-208 (1979).
Macfaddin, J.F. Biochemical tests for identification of medical bacteria. 2nd Ed, Williams & Wilkins, Baltimore, London, Sydney), p. 431 (1980).
Akit J., D. G. Cooper, K.I. Manninen, J.E. Zejic. Investigation of Potential biosurfactant production among phytopathogenic corynebacteria and related soil microbes. Current Microbiology 6:145-150 (1981).
Cooper, D.G & B.G. Goldenberg. Surface active against from two Bacillus species. Applied Environmental Microbiology 53(2):224-229 (1987).
Syldatk, C., S. Lang, U. Matulovic, F. Wagner. Production of four interfacial active rhamnolipid from n-alkanes or glycerol from resting cells of Pseudomonas species DSM 2874. Zeitschrift für Naturforschung C. 40:61-67 (1985).
Mulligan, C.N., D.G. Cooper, R. J. Neufeld, Selection of microbes producing biosurfactant in media without Hydrocarbons. Journal of
Fermentation Technology 62(4):311-314 (1984).
Mulligan, C .N., S. K. Sharma, A. Mudhoo. Biosurfactants: Research Trends and Applications. https://books google.com/books?isbn=1466518235(2014).
Cooper, D.G., J. Akit, N. Kosaric. Surface activity of the cells and extracellular lipids of Corynebacterium fascians CF 15. Journal Fermentation Technology 60:19-24 (1982).
Miller, G.L. Use of DinitrosaIicyIic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry 31(3):426-428 (1959).
Brandl, H. R. A., R. W. L. Gross, & R. C. Fuller. Pseudomonas oleovorans as a source of Poly (p-Hydroxyalkanaotes) for Potential Applications as Biodegradable Polyesters. Applied and Environmental Microbiology 54: 1977-1982 (1988).
Cooper, D.G., J. Akit, N. Kosaric. Surface activity of the cells and extracellular lipids of Corynebacterium fascians CF 15. Journal of Fermentation Technology 60:19-24. (1982).
Yalçin, E & A. Ergene. Preliminary characterization of biosurfactants produced by microorganisms isolated from refinery wastewaters. Environmental Technology 31(2):225-32 (2010).
Singer, M.E. Microbial biosurfactants. In: Microbes and Oil Recovery. Zajic, J.E & E.C. Donaldson (Ed.), Bio- resource Publications, El-Paso, TX, USA, p. 19-38 (1985).
Youssef, N.H., K.E. Duncana, D.P. Naglea, N. Kristen, K.N. Savagea et al. Comparison of methods to detect biosurfactant production by diverse microorganisms. Journal of Microbiological Methods 56:339-347 (2004).
Zajic, J.E and W. Seffens. Biosurfactant. CRC Critical Review Biotechnology1:187-107 (1984).
Tuleva, B.K., G.R. Ivanovb. Biosurfactant Production by a New Pseudomonas putida Strain. Zeitschrift für Naturforschung 57C:356-360 (2002).
Abouseoud M., R. Maachi, A. Amrane. Biosurfactant Production from olive oil by Pseudomonas fluorescens. Communicating Current
Research and Educational Topics and Trends in Applied Microbiology. A. Méndez- Vilas (Ed.) FORMATEX p.340-347 (2007).
Fa´tima, M.B., A. Fla´vio, C. de Oliveira, C.O. Benedict, T. F. Jr. William. Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiological Research 160:249-255 (2005).
Qazi, K & P. Rahman. Rhamnolipid biosurfactants: past, present & future scenario of global Market. Frontier of Microbiology 5:454 (2014).
Steinbu¨chel, A & B. Fu¨chtenbusch. Bacterial and other biological systems for polyester production. Trends in Biotechnology 16:419–427 (1998).
Nitschke, M., F.C. Cristina, G.M. Pastore. Selection of Microorganisms for Biosurfactant Production using Agro industrial wastes. Brazilian Journal Microbiology. 35:81-85 (2004).
Das, M., S.K. Das, R.K. Mukherjee. Surface active properties of the culture filtrates of a Micrococcus species grown on n-alkanes and sugars. Bio-resource Technology. 63(3):231–235 (1998).
Maneerat, S & K. Phetrong. Isolation of biosurfactantproducing marine bacteria and characteristics of selected biosurfactant. Songklanakarin Journal of Science & Technology 29(3):781-791 (2007).
Tuleva, B.K & G.R. Ivanovb. Biosurfactant Production by a New Pseudomonas putida Strain. Zeitschrift für Naturforschung 57C: 356-360 (2009).
Hamed, S.B., L. Smii, A. Ghram, A. Maaroufi. Screening of potential biosurfactant producing bacteria isolated from seawater biofilm. African Journal Biotechnology 11(77):14153-58 (2012).
Silva, E.J., N.M. Rocha e Silva, R.D. Rufino, J.M. Luna, R.O. Silva, et al. Characterization of a biosurfactant produced by Pseudomonas
cepacia CCT6659 in the presence of industrial wastes and its application in the biodegradation of hydrophobic compounds in soil. Colloids Surface B: Biointerfaces, 117:36-41 (2014).
Published
How to Cite
Issue
Section
License
Creative Commons Attribution (CC BY). Allows users to: copy the article and distribute; abstracts, create extracts, and other revised versions, adaptations or derivative works of or from an article (such as a translation); include in a collective work (such as an anthology); and text or data mine the article. These uses are permitted even for commercial purposes, provided the user: includes a link to the license; indicates if changes were made; gives appropriate credit to the author(s) (with a link to the formal publication through the relevant DOI); and does not represent the author(s) as endorsing the adaptation of the article or modify the article in such a way as to damage the authors' honor or reputation.
