Synthesis and In vitro Evaluation of Doxorubicin loaded Polymeric Nanoparticles on Cancer Cells

Synthesis and In vitro Evaluation of Doxorubicin loaded Polymeric Nanoparticles

Authors

  • Nimra Batool Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
  • Tayyaba Saif Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
  • Tahira Anwar Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
  • Sajjad Ullah University Institute of Medical Lab Technology, The University of Lahore, Pakistan
  • Sikandar Hayat Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
  • Malik Ihsan Ullah Khan Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan

DOI:

https://doi.org/10.53560/PPASB(61-sp1)1005

Keywords:

Cancer, Chemotherapy, Doxorubicin, Drug delivery, PLGA nanoparticles

Abstract

Cancer is the second biggest mortality rate globally. Most of anti-cancer drugs are hydrophobic and when they are administered in the body, they get clear from the blood. That’s why polymeric nanoparticles (NPs) have been used for delivering anti-cancer drugs to targeted sites. Biodegradable and self-assembled nature, PEG-PLGA has been used as a nanocarrier for biomedical applications. We developed PEG-PLGA NP for the doxorubicin (DOX) delivery to cancerous cells. The successful PEG-PLGA synthesis was confirmed by its 1 H NMR spectrum. All NPs displayed individual spherical morphology and 100 nm size range with -18.5mV zeta potential. Drug release profile showed DOX had sustained release pattern from DOX@NPs. In vitro, MTT assay and apoptosis analysis revealed that low-dose DOX@NPs exhibited more toxic effects on cancerous cells as compared to DOX alone. Overall results demonstrate that polymeric-based nanosystems increase the efficacy of DOX on cancer cells.

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Published

2024-04-24

How to Cite

Nimra Batool, Tayyaba Saif, Tahira Anwar, Sajjad Ullah, Sikandar Hayat, & Malik Ihsan Ullah Khan. (2024). Synthesis and In vitro Evaluation of Doxorubicin loaded Polymeric Nanoparticles on Cancer Cells: Synthesis and In vitro Evaluation of Doxorubicin loaded Polymeric Nanoparticles. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 61(S). https://doi.org/10.53560/PPASB(61-sp1)1005

Issue

Vol. 61 No. S (2024): Special Issue on Pathogen Transmission Beyond Borders: Understanding the Complexities of Cross-species Infectious Diseases

Section

Research Articles

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