Betalain as a Food Colorant: its Sources, Chemistry and Health Benefits
Chemistry of Betalain and its Role as Food Colorant
Keywords:
Food color, Betalain, Betacyanin, Betaxanthins, Pharmacological, HealthAbstract
Food colors are essential ingredients of modern processed foods. Betalain can be used as a natural color source for various foods due to its nutritional and safety value. This natural and water soluble dye occurs either as red-violet betacyanin or the yellow-orange betaxanthins. Betalain plays a vital role in human health due to its pharmacological activities such as antioxidant, anti-lipidemic, antimicrobial, antitumor, antiviral and anticancer potential. There are many eatable sources of betalain e.g., Opuntia stricta (cactus fig), Opuntia ficus-indica (barbary fig), Beta vulgarius (red beet), Amaranathus tricolor (Amaranath), Celosia argentea (silver cock’s comb) and Chenopodium quinoa Wild. Red beetroot provides carotenoids, glycine, betanin, saponins, betacyanin, folates, polyphenols and flavonoids. Amaranthus is an important source of vitamin C, vitamin B, soluble fiber, betacyanin and shows significant antimalarial and antimicrobial activity. Celosia argentea Linn provides carbohydrates, vitamins, dietary minerals and high amount of betalain. Chenopodium quinoa contains high content of vitamins and minerals. Betalain of Opuntia ficus-indica fruits (barbary fig) possesses strong anti-proliferative activity. Extract of prickly pear (cactus fig) pulp which contains betalain, can act as an antibiotic agent in cases of wounds, digestive and urinary tract infections and inflammations. It also has significant inhibitory effect against humanoid ovarian tumor and cervical cancer cells. Optimum pH for maximum stability of betanin lies between pH 5.5 and 5.8. Betalain stability is affected by long term exposure to oxygen/air, light in the presence of oxygen, high temperature and water activity (aw) but it is highly stable in the presence of low moisture.
References
Chattopadhyay, P., S. Chatterjee & S.K. Sen Biotechnological potential of natural food grade biocolorants. African Journal of Biotechnology, 7(17): 2972-2985 (2008).
Rymbai, H., R. Sharma &M. Srivastav, Bio-colorants and its implications in health and food industry–a review. International Journal of Pharmtech Research, 3(4): 2228-2244 (2011).
De Azeredo, H. M. C., A. C. Pereira, A. C. R. De Souza, S. T. Gouveia & K. C. B. Mendes, Study on efficiency of betacyanin extraction from red beetroots. International Journal of Food Science & Technology, 44(12): 2464-2469 (2009).
Benson L. Family 7 Amaranthaceae (amaranth family). In: Benson L (Ed.), Plant classification. D.C. Heath and Company, Boston. 196-198 (1957).
Aberoumand, A. A review article on edible pigments properties and sources as natural biocolorants in foodstuff and food industry. World Journal of Dairy and Food Sciences, 6(1): 71-78 (2011).
Tanaka, Y., N. Sasaki & A. Ohmiya. Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids. The Plant Journal, 54(4): 733-749 (2008).
Shamina, A., K. Shiva & V. Parthasarathy. Food colors of plant origin. CAB Reviews: Perspectives in Agriculture, Veterinary Science. Nutrition and Natural Resources, 2(1-25): (2007).
Rivera-Madrid, R., M. Aguilar-Espinosa, Y. Cárdenas-Conejo & L. E. Garza-Caligaris. Carotenoid derivates in achiote (Bixa orellana) seeds: synthesis and health promoting properties. Frontiers in plant science, 7, 1406 (2016).
Santos-Buelga, C., N. Mateus & V. De Freitas. Anthocyanins. Plant pigments and beyond. ACS Publications (2014).
Adeel, S., F.-U. Rehman, S. Rafi, K. M. Zia & M. Zuber. Environmentally Friendly Plant-Based Natural Dyes: Extraction Methodology and Applications, in: Plant and Human Health, Springer (2019).
Esquivel, P. Betalains, in Handbook on Natural Pigments in Food and Beverages. Elsevier. (2016).
Esatbeyoglu, T., A. E. Wagner, V. B. Schini-Kerth & G. Rimbach. Betanin—A food colorant with biological activity. Molecular Nutrition & Food Research 59(1): 36-47 (2015).
Strack, D., T. Vogt, &W. Schliemann. Recent advances in betalain research. Phytochemistry, 62: 247–269 (2003).
Azeredo, H. M. Betalains: properties, sources, applications, and stability–a review. International Journal of Food Science & Technology, 44(12): 2365-2376 (2009).
Šaponjac, V. T., J. Čanadanović-Brunet, G. Ćetković, M. Jakišić, S. Djilas, J. Vulić & S. Stajčić. Encapsulation of beetroot pomace extract: RSM optimization, storage and gastrointestinal stability. Molecules, 21(5): 584 (2016).
Choo, W. S. Betalains: application in functional foods.In book: Bioactive Molecules in Food. In: Mérillon J. M., Ramawat K. (eds). Bioactive Molecules in Food. Reference Series in Phytochemistry. Springer, Cham (2017).
Kezi, J. & J. Sumathy. Betalain-a boon to the food industry. Discovery, 20(63): 51-58 (2014).
Vieira Teixeira da Silva, D., D. dos Santos Baião, F. de Oliveira Silva, G. Alves, D. Perrone, E. Mere Del Aguila & V. M Flosi Paschoalin. Betanin, a Natural Food Additive: Stability, Bioavailability, Antioxidant and Preservative Ability Assessments. Molecules, 24(3): E458 (2019).
Li, G., X. Meng, M. Zhu & Z. Li, Research Progress of Betalain in Response to Adverse Stresses and Evolutionary Relationship Compared with Anthocyanin. Molecules, 24(17): 3078 (2019).
Gengatharan, A., G. A. Dykes & W. S. Choo. Natural plant pigments with potential application in functional and food. Food Science and Technology, 64: 645-649 (2015).
Gasztonyi, M. N., H. Daood, M. T. Hajos & P. Biacs. Comparison of red beet (Beta Vularis Var conditiva) varieties on the basis of their pigment components. Journal of the Science of Food and Agriculture, 81: 932- 933 (2001).
Singh, B. & B.S. Hathan. Chemical composition, functional properties and processing of Beetroot—a review. International Journal of Scientific & Engineering Research, 5(1): 679-684 (2014).
Gentile, C., L. Tesoriere, M. Allegra, M. A. Livrea, &P. Dalessio. Antioxidant betalains from Cactus Pear (Opuntia ficus-indica) inhibit endothelial ICAM-1 expression. Annals of the New York Academy of Sciences, 1028(1): 481-486 (2004).
Miguel, M. G. Betalains in Some Species of the Amaranthaceae Family: A Review. Antioxidants, 7(4): 53(2018).
Surse, S., B. Shrivastava, P. Sharma, P. Gide & S. Attar. Celosia cristata: Potent pharmacotherapeutic herb—A review. International journal of pharmaceutical and phytopharmacological research, 3: 444-446 (2014).
Schliemann, W., Y. Cai, T. Degenkolb, J. Schmidt & H. Corke, Betalains of Celosia argentea. Phytochemistry, 58(1): 159-165 (2001).
Ravichandran, K., R. Palaniraj, N. M. M. T. Saw, A. M. Gabr, A. R. Ahmed, D. Knorr & I. Smetanska. Effects of different encapsulation agents and drying process on stability of betalains extract. Journal of Food Science and Technology, 51(9): 2216-2221 (2014).
Stintzing, F. C. & R. Carle. Functional properties of anthocyanins and betalains in plants, food, and in human nutrition. Trends in Food Science & Technology, 15(1): 19-38 (2004).
Gandía-Herrero, F., J. Escribano & F. García-Carmona, Biological activities of plant pigments betalains. Critical reviews in food science and nutrition, 56(6): 937-945 (2016).
Scotter, M. J. Emerging and persistent issues with artificial food colors: natural color additives as alternatives to synthetic colors in food and drink. Quality Assurance and Safety of Crops & Foods, 3(1): 28-39 (2011).
Cai, Y. & H. Corke. Amaranthus betacyanin pigments applied in model food systems. Journal of Food Science, 64: 869–873(1999).
Bolkent, S., R. Yanardag, A. Tabakoğlu-Oğuz & O. Ozsoy-Saçan. Effects of chard (Beta vulgaris L. var. cicla) extract on pancreatic B cells in streptozotocin-diabetic rats: a morphological and biochemical study. Journal of Ethnopharmacology, 73(1): 251-259 (2000).
Herbach, K. M., F. C. Stintzing & R. Carle. Betalain stability and degradation—structural and chromatic aspects. Journal of Food Science, 71(4): R41-R50 (2006).
Castellar, R., J. M. Obon, M. Alacid & J. A. Fernández-López. Color properties and stability of betacyanins from Opuntia fruits. Journal of Agricultural and Food Chemistry, 51(9): 2772-2776 (2003).
Huang, A.S.& J.V. Elbe. Effect of pH on the degradation and regeneration of betanin. Journal of Food Science, 52(6): 1689-1693 (1987).
Elbe, J. H., S. J. Schwartz & B.E. Hildenbrand. Loss and regeneration of betacyanin pigments during processing of red beets. Journal of Food Science, 46(6): 1713-1715 (1981).
Cohen, E. & I. Saguy. Effect of water activity and moisture content on the stability of beet powder pigments. Journal of Food Science, 48(3): 703-707 (1983).
Havlíková, L., K. Miková & V. Kyzlink. Heat stability of betacyanins. Zeitschrift für Lebensmittel-Untersuchung und Forschung, 177(4): 247-250 (1983).
Wettasinghe, M., B. Bolling, L. Plhak, Xiao, H. and K. Parkin. Phase II enzyme-inducing and antioxidant activities of beetroot (Beta vulgaris L.). Extracts from phenotypes of different pigmentation. Journal of Agricultural and Food Chemistry, 50: 6704-6709 (2002).
Naseer, S., S. Hussain, N. Naeem, M. Pervaiz & M. Rahman. The phytochemistry and medicinal value of Psidium guajava (guava). Clinical Phytoscience, 4:1-8 (2018).
Rehman, A., S. Hussain, M. Javed, Z. Ali, H. Rehman, T. G. Shahzady, A. Zahra. Chemical composition and remedial Perspectives of hippophae rhamnoides linn. Postepy Biologii Komorki, 45:199-210 (2018).
Naseer, S., S. Hussain, and Z. Zahid. Nutritional and antioxidant potential of common vegetables in Pakistan. RADS Journal of Biological Research & Applied Sciences, 10(1): 36-40 (2019).
Gliszczyńska-Świgło, A., H. Szymusiak & P. Malinowska. Betanin, the main pigment of red beet: Molecular origin of its exceptionally high free radical-scavenging activity. Food additives and contaminants, 23(11): 1079-1087 (2006).
Tahir, N.I., S. Hussain, M. Javed, H. Rehman, T. G. Shahzady, B. Parveen & K.G. Ali. Nature of aflatoxins: Their extraction, analysis, and control. Journal of Food Safety, 38: (2018). doi.org/10.1111/jfs.12561.
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